In the context of global warming and continuous high temperatures in the northern part of China in summer, the mortality rate of Strongylocentrotus intermedius through the summer reaches 70–80 %. The protein regulatory mechanism of S. intermedius in response to high temperature stress is still unclear. In order to investigate the protein expression of S. intermedius under high temperature stress, the study was conducted with the high-temperature resistant strain of S. intermedius and the control group of S. intermedius. Tandem Mass Tag (TMT) tagging technique was applied to resolve the protein expression profile of S. intermedius in response to high temperature stress. The results showed that, compared to 15 °C，136 DEPs were screened in high-temperature resistant strain groups of S. intermedius under high temperature stress and 87 DEPs were screened in the control group of S. intermedius. There were 33 common differential proteins in the two groups, such as APOLP, HSP 70, CDC37 and CALM. Further Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analyses revealed that the up-regulated proteins CALM and HSP70 are significantly enriched in the “Phosphatidylinositol signaling system” and “Protein processing in endoplasmic reticulum” in heat-tolerant S. intermedius strains under high temperature stress. The control group of S. intermedius DEPs were significantly enriched in protein processing in the endoplasmic reticulum. These results provide a theoretical basis for the molecular mechanism of sea urchin heat tolerance and fundamental data for sea urchin selection and breeding for high temperature tolerance.

在全球气候变暖、我国北方夏季持续高温的背景下，中间圆甲鱼整个夏季的死亡率高达70%~80%。S. intermedius响应高温胁迫的蛋白质调控机制仍不清楚。为了探讨高温胁迫下S. intermedius的蛋白表达情况，以耐高温菌株S. intermedius和对照组S. intermedius进行研究。应用串联质量标签 (TMT) 标记技术解析中间链霉菌响应高温胁迫的蛋白质表达谱。结果表明，与15 ℃相比，高温胁迫下的耐高温菌株组中筛选出136个DEP ，对照组中筛选出87个DEP 。两组共有33个差异蛋白，如APOLP、HSP 70、 CDC37、CALM。进一步的京都基因与基因组百科全书（KEGG）通路分析显示，在高温条件下耐热中间链霉菌菌株的“磷脂酰肌醇信号系统”和“内质网蛋白质加工”中上调的蛋白CALM和HSP70显着富集。温度应力。对照组的S. intermedius DEP 在内质网中的蛋白质加工中显着富集。该研究结果为海胆耐热性的分子机制提供了理论依据，为海胆耐高温选育提供了基础数据。