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First report of Colletotrichum gloeosporioides causing severe anthracnose on Litsea deccanensis seedlings in India
Physiological and Molecular Plant Pathology ( IF 2.7 ) Pub Date : 2023-12-26 , DOI: 10.1016/j.pmpp.2023.102212
S. Praveena , Shambhu Kumar , P.A. Jose , K.T. Mufeeda

Litsea deccanensis, commonly known as Jiggat, finds commercial use in India for the production of agarbatti or incense sticks, with its stem bark serving as a crucial raw material. The plant seedlings are important for conservation efforts, biodiversity, and potential uses in medicine, horticulture, and research. Within the campus nursery of the Institute, distinct signs of anthracnose were observed in the seedlings of L. deccanensis. The incidence of this disease was documented at 50 % among the hundred assessed seedlings maintained in the KFRI nursery. Through rigorous isolation, purification, and analysis, the responsible pathogen was identified as Colletotrichum sp. Phylogenetic analysis of concatenated sequence alignment of Internal Transcribed Spacer (ITS) region, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and beta-tubulin (TUB2) genes supported by cultural morphological characteristics revealed Colletotrichum gloeosporioides as causal agent. To affirm the pathogenic nature of the fungus, pathogenicity tests were conducted, both in controlled environments and within the living plant, supported by subsequent re-isolation and identification of the pathogen, in accordance with Koch's postulates. To the best of our knowledge, this is the first report of anthracnose caused by C. gloeosporioides on Litsea deccanensis in India.



中文翻译:

印度首次报告胶孢炭疽菌对德干木姜子幼苗造成严重炭疽病

Litsea deccanensis,俗称 Jiggat,在印度商业用途为生产 agarbatti 或线香,其茎皮是重要的原材料。植物幼苗对于保护工作、生物多样性以及在医学、园艺和研究中的潜在用途非常重要。在该研究所的校园苗圃内,在德干乳杆菌的幼苗中观察到明显的炭疽病迹象。据记录,在 KFRI 苗圃中饲养的 100 个经过评估的幼苗中,这种疾病的发病率为 50%。经过严格的分离、纯化和分析,确定病原菌为炭疽病菌(Colletotrichum sp.)。对内部转录间隔区 (ITS) 区域、3-磷酸甘油醛脱氢酶 (GAPDH) 和 β-微管蛋白 (TUB2) 基因的串联序列比对进行系统发育分析,并得到培养形态学特征的支持,结果表明胶孢炭疽菌致病因子。为了确认真菌的致病性质,在受控环境和活植物内进行了致病性测试,并根据科赫的假设对病原体进行了随后的重新分离和鉴定。据我们所知,这是印度首次报道由胶孢炭疽菌引起的十干木姜子炭疽病。

更新日期:2023-12-30
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