Background:: Umbilical cord mesenchymal stem cells (UC-MSCs) are increasingly being utilized for immune-related disease therapies due to their low immunogenicity. However, the primary culture of UC-MSCs requires the supplementation of serum in the growth medium, which has posed a challenge due to ethical issues related to the collection method of the fetal bovine serum (FBS) that is routinely used in cell culture. Aim:: In order to address this, the purpose of this research was to assess the effectiveness of adult bovine serum (ABS) as a different and more affordable source of serum for the in-vitro cultivation of UC-MSCs. UC-MSCs were isolated from the umbilical cord of Wharton’s jelly of cow immediately after birth, by digestion with Collagenase type I. Method:: ABS was collected from fresh bovine sources and heat-inactivated. The morphology of UC-MSCs was observed under an inverted microscope, and growth patterns, proliferative index, and doubling time were calculated every two days to compare the efficacy of ABS with FBS. Immunocytochemistry for specific markers was also conducted on the MSCs. Result:: The results showed a notable difference in morphology, growth rate, population doubling, and proliferative index between ABS and FBS. Conclusion:: Intriguingly, ABS proved to be an effective supplement in the growth medium for expanding UC-MSCs in vitro, providing a viable alternative to FBS. conclusion: ABS proved to be an effective supplement in the growth medium for expanding UC-MSCs in-vitro, providing a viable alternative to fetal bovine serum other: Stem cells, Adult bovine serum, fetal bovine serum, viability, mesenchymal Stem Cells, umbilical cord.

中文翻译：

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使用成年牛血清体外增强间充质干细胞

背景：脐带间充质干细胞（UC-MSC）由于其低免疫原性而越来越多地用于免疫相关疾病的治疗。然而，UC-MSCs的原代培养需要在生长培养基中补充血清，由于细胞培养中常规使用的胎牛血清（FBS）的收集方法相关的伦理问题，这提出了挑战。目的：为了解决这个问题，本研究的目的是评估成年牛血清 (ABS) 作为一种不同且更实惠的血清来源用于 UC-MSC 体外培养的有效性。UC-MSCs是在牛出生后立即通过I型胶原酶消化从沃顿氏胶的脐带中分离出的UC-MSC。方法：：从新鲜牛源中收集ABS并热灭活。在倒置显微镜下观察UC-MSCs的形态，每两天计算一次生长模式、增殖指数和倍增时间，以比较ABS与FBS的功效。还在 MSC 上进行了特定标记物的免疫细胞化学分析。结果：结果显示ABS和FBS在形态、生长速度、群体倍增和增殖指数方面存在显着差异。结论:: 有趣的是，ABS 被证明是体外扩增 UC-MSC 生长培养基的有效补充物，提供了 FBS 的可行替代品。结论：ABS 被证明是体外扩增 UC-MSC 生长培养基的有效补充，为胎牛血清提供了可行的替代品其他：干细胞、成体牛血清、胎牛血清、活力、间充质干细胞、脐带血绳索。