Abstract

Background

Plukenetia volubilis Linneo is an oleaginous plant belonging to the family Euphorbiaceae. Due to its seeds containing a high content of edible oil and rich in vitamins, P. volubilis is cultivated as an economical plant worldwide. However, the cultivation and growth of P. volubilis is challenged by phytopathogen invasion leading to production loss.

Methods

In the current study, we tested the pathogenicity of fungal pathogens isolated from root rot infected P. volubilis plant tissues by inoculating them into healthy P. volubilis seedlings. Metagenomic sequencing was used to assess the shift in the fungal community of P. volubilis rhizosphere soil after root rot infection.

Results

Four Fusarium isolates and two Rhizopus isolates were found to be root rot causative agents of P. volubilis as they induced typical root rot symptoms in healthy seedlings. The metagenomic sequencing data showed that root rot infection altered the rhizosphere fungal community. In root rot infected soil, the richness and diversity indices increased or decreased depending on pathogens. The four most abundant phyla across all samples were Ascomycota, Glomeromycota, Basidiomycota, and Mortierellomycota. In infected soil, the relative abundance of each phylum increased or decreased depending on the pathogen and functional taxonomic classification.

Conclusions

Based on our results, we concluded that Fusarium and Rhizopus species cause root rot infection of P. volubilis. In root rot infected P. volubilis, the shift in the rhizosphere fungal community was pathogen-dependent. These findings may serve as a key point for a future study on the biocontrol of root rot of P. volubilis.

中文翻译：

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根际土壤真菌群落的变化与由镰刀菌和根霉引起的 Plukenetia volubilis Linneo 根腐病感染相关

摘要

背景

Plukenetia volubilis Linneo 是属于大戟科的油质植物。由于其种子含有高含量的食用油和丰富的维生素，油菜籽在世界范围内作为经济植物栽培。然而， P. volubilis的栽培和生长受到植物病原体入侵的挑战，导致产量损失。

方法

在当前的研究中，我们通过将真菌病原体接种到健康的云杉幼苗中，测试了从感染根腐病的云杉植物组织中分离出的真菌病原体的致病性。使用宏基因组测序来评估根腐病感染后P. volubilis根际土壤真菌群落的变化。

结果

四种镰刀菌分离株和两种根霉分离株被发现是P. volubilis根腐病的病原体，因为它们会在健康幼苗中诱导典型的根腐病症状。宏基因组测序数据表明根腐病感染改变了根际真菌群落。在根腐病感染的土壤中，丰富度和多样性指数根据病原体而增加或减少。所有样本中最丰富的四个门是子囊菌门、球囊菌门、担子菌门和被孢霉门。在受感染的土壤中，每个门的相对丰度根据病原体和功能分类学分类而增加或减少。

结论

根据我们的结果，我们得出结论，镰刀菌和根霉属引起 P. volubilis 根腐病感染。在根腐病感染的P. volubilis中，根际真菌群落的变化是病原体依赖性的。这些发现可能成为今后研究P. volubilis根腐病生物防治的关键点。