The objective of this study was to investigate the mRNA expression of insulin-like growth factor-1 (IGF-1), pro-inflammatory cytokines (IL-1β, IL-6, IL-18, and TNF-α), serum immunoglobulin profiles (IgG and IgM), and lipid peroxidation status (MDA) in relation to pro-inflammatory cytokines. A case-controlled, prospective, and observational investigation was completed on 85 calves. Total RNA was isolated from whole blood samples of both the SIRS and healthy calves, followed by reverse transcription into cDNA. The resulting cDNAs were mixed with iTaq Universal SYBR Green Supermix and primers specific to the relevant genes using the Rotor-Gene Q instrument. After the reaction was completed, gene expressions were normalised against β-actin using the 2^-ΔΔCT method. The mRNA levels of pro-inflammatory cytokines namely (IL-1β [SIRS: 2.15 ± 0.55, Control: 1.13 ± 0.62; P = 0.001], IL-6 [SIRS: 2.82 ± 0.52, Control: 0.91 ± 0.11; P < 0.001], IL-18 [SIRS: 1.92 ± 0.41, Control: 0.99 ± 0.13; P < 0.001], and TNF-α [SIRS: 2.59 ± 0.28, Control: 0.93 ± 0.09; P < 0.001]) and IGF-1 (SIRS: 3.55 ± 0.55, Control: 0.91 ± 0.15; P < 0.001) were up-regulated in calves with SIRS, while serum IgG (SIRS: 4.16 ± 0.26, Control: 1.73 ± 0.17; P < 0.001), IgM (SIRS: 1.55 ± 0.11, Control: 1.09 ± 0.13; P < 0.001), and MDA levels (SIRS: 41.12 ± 3.48, Control: 3.76 ± 0.81; P < 0.001) increased significantly in these calves. Furthermore, significant (P < 0.01) positive correlations were found in calves with SIRS in relation to the expression levels of IL-1β, IL-6, IL-18, TNF-α, IGF-1, serum immunoglobulins, and MDA levels. These results suggest that IGF-1 could be a valuable pro-inflammatory marker, considering its high positive correlation with the expression levels of pro-inflammatory cytokines (IL-1β, IL-6, IL-18, and TNF-α) and markers (MDA, IgG, and IgM) in calves with SIRS.

本研究的目的是调查胰岛素样生长因子-1 (IGF-1)、促炎细胞因子（IL-1β、IL-6、IL-18 和 TNF-α）、血清免疫球蛋白的 mRNA 表达与促炎细胞因子相关的概况（IgG 和 IgM）和脂质过氧化状态（MDA）。对 85 头小牛完成了病例对照、前瞻性和观察性调查。从 SIRS 和健康小牛的全血样本中分离出总 RNA，然后逆转录为 cDNA。使用 Rotor-Gene Q 仪器将所得 cDNA 与 iTaq Universal SYBR Green Supermix 和相关基因特异的引物混合。反应完成后，使用 2 ^-ΔΔCT方法针对 β-肌动蛋白对基因表达进行标准化。促炎细胞因子的mRNA水平即(IL-1β [SIRS: 2.15 ± 0.55, Control: 1.13 ± 0.62; P = 0.001], IL-6 [SIRS: 2.82 ± 0.52, Control: 0.91 ± 0.11; P < 0.001 ]、IL-18 [SIRS：1.92 ± 0.41，对照：0.99 ± 0.13；P < 0.001]、TNF-α [SIRS：2.59 ± 0.28，对照：0.93 ± 0.09；P < 0.001]）和 IGF-1（ SIRS 犊牛中 SIRS：3.55 ± 0.55，对照：0.91 ± 0.15；P < 0.001）上调，而血清 IgG（SIRS：4.16 ± 0.26，对照：1.73 ± 0.17；P < 0.001）、IgM（SIRS：这些犊牛的 MDA 水平（SIRS：41.12 ± 3.48，对照：3.76 ± 0.81；P < 0.001）显着增加（1.55 ± 0.11，对照：1.09 ± 0.13；P < 0.001）。此外，SIRS 犊牛中 IL-1β、IL-6、IL-18、TNF-α、IGF-1、血清免疫球蛋白和 MDA 水平的表达水平呈显着正相关（P < 0.01）。这些结果表明，考虑到 IGF-1 与促炎细胞因子（IL-1β、IL-6、IL-18 和 TNF-α）和标记物的表达水平高度正相关，IGF-1 可能是一种有价值的促炎标记物。 （MDA、IgG 和 IgM）患有 SIRS 的小牛。