At its most fundamental level, life is a collection of synchronized cellular processes driven by interactions among biomolecules. Proximity labeling has emerged as a powerful technique to capture these interactions in native settings, revealing previously unexplored elements of biology. This review highlights recent developments in proximity labeling, focusing on methods that push the fundamental technologies beyond the classic bait-prey paradigm, such as RNA–protein interactions, ligand/small-molecule–protein interactions, cell surface protein interactions, and subcellular protein trafficking. The advancement of proximity labeling methods to address different biological problems will accelerate our understanding of the complex biological systems that make up life.

中文翻译：

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使用邻近标记获得新的生物学见解的创造性方法

在最基本的层面上，生命是由生物分子之间的相互作用驱动的同步细胞过程的集合。邻近标记已成为捕获自然环境中这些相互作用的强大技术，揭示了以前未探索的生物学元素。这篇综述重点介绍了邻近标记的最新进展，重点关注推动基本技术超越经典诱饵-猎物范式的方法，例如RNA-蛋白质相互作用、配体/小分子-蛋白质相互作用、细胞表面蛋白质相互作用和亚细胞蛋白质运输。解决不同生物问题的邻近标记方法的进步将加速我们对构成生命的复杂生物系统的理解。