Meat quality is a critical aspect of pig breeding. In addition to genetics, meat quality is also influenced by nutritional and environmental factors. In this study, three pig breeds, Shengxianhua, Jiaxing, and Qinglian Black (SXH, JXB and QLB), were used as experimental animals. Transcriptional analysis was performed on the longissimus thoracis (LT) muscle to investigate variations in intramuscular fat (IMF), inosine monophosphate (IMP), amino acids, and muscle fiber morphology across different breeds. Ingenuity canonical pathway analysis (IPA) identified biological processes and key driver genes related to metabolism and muscle development. Additionally, weighted gene co-expression network analysis (WGCNA) revealed gene modules associated with IMP. KEGG and GO analyses identified specific biological processes and signaling pathways related to IMP, including the Oxidative Phosphorylation pathway and rRNA Metabolic Processes. These findings provide novel insights into the molecular regulatory mechanisms underlying meat quality variations among pig breeds.

肉质是养猪业的一个重要方面。除了遗传因素外，肉质还受到营养和环境因素的影响。本研究以生鲜花、嘉兴和青莲黑（SXH、JXB和QLB）三个猪品种为实验动物。对胸最长肌 (LT) 肌肉进行转录分析，以研究不同品种的肌内脂肪 (IMF)、肌苷一磷酸 (IMP)、氨基酸和肌纤维形态的变化。Ingenuity 经典路径分析 (IPA) 确定了与新陈代谢和肌肉发育相关的生物过程和关键驱动基因。此外，加权基因共表达网络分析 (WGCNA) 揭示了与 IMP 相关的基因模块。KEGG 和 GO 分析确定了与 IMP 相关的特定生物过程和信号传导途径，包括氧化磷酸化途径和 rRNA 代谢过程。这些发现为猪品种间肉质差异的分子调控机制提供了新的见解。