Escherichia coli (E. coli) causes various infections in humans and animals. The biofilm-forming ability of E. coli has increased antimicrobial resistance and capacity to cause recurrent and chronic infections. This study determined the biofilm-forming ability of E. coli isolated from extraintestinal infections of humans, chickens, and dogs in relation to the phylogroup, type of infection, and antibiotic resistance. Isolates from chickens showed significantly higher biofilm-forming ability compared to those causing urinary tract infections in humans (p = 0.0001). Further, isolates belonging to phylogroup B1 displayed a higher likelihood to form biofilms. Resistance to ciprofloxacin and trimethoprim-sulfamethoxazole was positively correlated with biofilm-forming ability. Harbouring plasmid-mediated quinolone resistance gene, qnrS was also positively correlated with biofilm formation. This study provides insight into factors such as phylogroup and the type of infections that could enhance biofilm formation, as well as genotypic and phenotypic antibiotic resistance that could correlate with the ability to form biofilms

大肠杆菌( E. coli ) 会引起人类和动物的各种感染。大肠杆菌的生物膜形成能力增加了抗菌素耐药性和引起复发性和慢性感染的能力。这项研究确定了从人类、鸡和狗的肠外感染中分离出的大肠杆菌的生物膜形成能力与系统群、感染类型和抗生素耐药性的关系。与引起人类尿路感染的分离物相比，来自鸡的分离物显示出显着更高的生物膜形成能力（p = 0.0001）。此外，属于系统群 B1 的分离株表现出更高的形成生物膜的可能性。对环丙沙星和甲氧苄啶-磺胺甲恶唑的耐药性与生物膜形成能力呈正相关。qnrS含有质粒介导的喹诺酮抗性基因，也与生物膜形成呈正相关。这项研究深入了解了可以增强生物膜形成的系统群和感染类型等因素，以及可能与生物膜形成能力相关的基因型和表型抗生素耐药性