Vascular aging is an important pathophysiological basis for the senescence of various organs and systems in the human body, and it is a common pathogenetic trigger for many chronic diseases in the elderly. The extracellular vesicles (EVs) from young and aged umbilical vein endothelial cells were isolated and identified by qPCR the differential expression levels of 47 mRNAs of genes closely related to aging in the two groups. There were significant differences in the expression levels of 18 genes (we noted upregulation in PLA2G12A, TP53BP1, CD144, PDE11A, FPGT, SERPINB4, POLD1, and PPFIBP2 and downregulation in ATP2C2, ROBO2, RRM2, GUCY1B1, NAT1-14, VEGFR2, WTAPP1, CD146, DMC1, and GRIK2). Subsequent qPCR identification of the above-mentioned genes in PBMCs and plasma-EVs from the various age groups revealed that the trend in expression levels in peripheral blood plasma-EVs of the different age groups was approximately the same as that in PBMCs. Of these mRNAs, the expression of four genes–PLA2G12A, TP53BP1, OPRL1, and KIAA0895–was commensurate with increasing age. In contradistinction, the expression trend of four genes (CREG1, PBX1, CD34, and SLIT2) was inversely proportional to the increase in age. Finally, by taking their intersection, we determined that the expression of TP53BP1 was upregulated with increasing human age and that CD34 and PBX1 were downregulated with increasing age. Our study indicates that human peripheral blood plasma-EV-derived TP53BP1, CD34, and PBX1 potentially comprise a noninvasive biomarker for assessing and predicting vascular aging.

中文翻译：

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来自人外周血的细胞外囊泡来源的 TP53BP1、CD34 和 PBX1 可作为评估和预测血管老化的潜在生物标志物

血管衰老是人体各器官、系统衰老的重要病理生理基础，是老年人多种慢性疾病的常见发病诱因。分离年轻和老年脐静脉内皮细胞的细胞外囊泡（EV），通过qPCR鉴定两组与衰老密切相关的基因的47个mRNA表达水平的差异。18个基因的表达水平存在显着差异（我们注意到PLA2G12A、TP53BP1、CD144、PDE11A、FPGT、SERPINB4、POLD1和PPFIBP2上调，ATP2C2、ROBO2、RRM2、GUCY1B1、NAT1-14、VEGFR2、WTAPP1下调、CD146、DMC1 和 GRIK2）。随后对各年龄组PBMCs和血浆-EVs中上述基因的qPCR鉴定显示，不同年龄组外周血血浆-EVs中的表达水平趋势与PBMCs大致相同。在这些 mRNA 中，四个基因——PLA2G12A、TP53BP1、OPRL1 和 KIAA0895——的表达与年龄的增加相称。相比之下，四个基因（CREG1、PBX1、CD34和SLIT2）的表达趋势与年龄的增加成反比。最后，通过取它们的交集，我们确定TP53BP1的表达随着人类年龄的增加而上调，而CD34和PBX1的表达随着年龄的增加而下调。我们的研究表明，人外周血浆 EV 衍生的 TP53BP1、CD34 和 PBX1 可能构成评估和预测血管老化的非侵入性生物标志物。