Introduction. The sperm DNA fragmentation index (DFI) is considered a valuable measure to assess male fertility, but the predictive value of DFI for the outcomes in assisted reproductive technology (ART) is still controversial. Therefore, this study is aimed at investigating the effect of requesting a DFI test or performing ART without DFI on the results observed in the embryology laboratory (number of embryos, fertilization rate, and embryo quality) after intracytoplasmic sperm injection (ICSI). Methods. This retrospective study was conducted on infertile men who underwent ICSI and were referred to the Avicenna Infertility and Recurrent Abortion Treatment Center in Tehran from 2019 to 2022. The samples were categorized into two groups: a case group with DFI measurement and a control group without DFI measurement. We conducted a comparative analysis of the embryology results between the two groups, focusing on parameters such as fertilization rate, number of embryos, and embryo quality. -tests and Mann–Whitney tests were used to conduct single variable analysis. Potential confounding effects were adjusted to use the multivariate linear and logistic regression. Results. Data analysis showed no significant statistical difference between the case group and the control group in terms of the number of embryos (95% confidence interval for the regression coefficient ), and embryo quality (95% confidence interval for ). There was no significant statistical difference between the two groups due to the fertilization rate (95% confidence interval for ), except for the variables of woman’s age and sperm count after ICSI, as determined by adjusted linear regression. Conclusions. Although DFI measurement is used to assess male infertility, its importance as a predictor for the embryology outcomes after ICSI requires further evaluation and the determination of a cut-off point for predicting results. This study was based on retrospectively collected DFI data, and prospective studies confirming the superiority of ICSI outcomes are necessary.

中文翻译：

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两组男性显微注射胚胎学结果比较（要求测量和不测量精子 DNA 碎片指数）

介绍。精子DNA碎片指数（DFI）被认为是评估男性生育能力的重要指标，但DFI对辅助生殖技术（ART）结果的预测价值仍存在争议。因此，本研究旨在调查胞浆内单精子注射（ICSI）后要求进行DFI测试或在没有DFI的情况下进行ART对胚胎学实验室观察到的结果（胚胎数量、受精率和胚胎质量）的影响。方法。这项回顾性研究针对 2019 年至 2022 年期间接受 ICSI 并转诊至德黑兰阿维森纳不孕不育和复发性流产治疗中心的不孕男性。样本分为两组：进行 DFI 测量的病例组和未进行 DFI 的对照组测量。我们对两组胚胎学结果进行了比较分析，重点关注受精率、胚胎数量和胚胎质量等参数。-使用检验和曼-惠特尼检验进行单变量分析。使用多元线性和逻辑回归调整潜在的混杂效应。结果。数据分析显示病例组与对照组胚胎数量无显着统计学差异（回归系数95%置信区间））和胚胎质量（95% 置信区间）。由于受精率（95％置信区间），两组之间没有显着的统计差异），除了通过调整的线性回归确定的女性年龄和 ICSI 后精子数量的变量。结论。尽管 DFI 测量用于评估男性不育症，但其作为 ICSI 后胚胎学结果预测指标的重要性需要进一步评估并确定预测结果的分界点。本研究基于回顾性收集的 DFI 数据，有必要进行前瞻性研究来证实 ICSI 结果的优越性。