Despite the high level of standardization of the intracytoplasmic sperm injection (ICSI) technique, there are some aspects that deserve special attention and should still be improved. The major drawback of the technique is its invasiveness, as during cytoplasmic aspiration different structures of the oocyte may be lost or damaged. This is partly because the microtools used in ICSI were not specially designed for assisted reproduction but for other medical–biological disciplines. In view of the above caveats, the aim of the study was to compare the results of ICSI with the traditional oocyte-holding pipette and the oocyte-holding pipette without aspiration (PiWA). In total, 155 patients and 1037 oocytes were included in the study. In each ICSI cycle, half of the oocytes were microinjected using a traditional holding pipette and the other half using a PiWA. In result, the PiWA technique produced a significant increase in the fertilization rate: 88.12% (95%CI: 84.62–90.92%); holding pipette: 73.33% (95%CI: 68.72–77.49%). Also, it produced a significant decrease in the embryo degeneration rate compared with the traditional holding pipette [PiWA: 2.07% (95%CI: 1.11–3.8%); holding pipette: 4.51% (95%CI: 3.06–6.59%)]. Pregnancy rate depended on the holding technique used, both in single embryo transfers (n = 59; χ2 = 4.608; P-value = 0.032) and double embryo transfers (n = 156; χ2 = 4.344; P-value = 0.037); with PiWA presenting a significantly higher pregnancy rate than the traditional holding technique. Based on current evidence and the present results, improvements should focus on decreasing the invasiveness of the microinjection itself by minimizing or avoiding aspiration and cytoplasmic disorganization, as is successfully achieved with PiWA.

尽管胞浆内单精子注射（ICSI）技术的标准化水平很高，但仍有一些方面值得特别关注并仍需改进。该技术的主要缺点是其侵入性，因为在细胞质抽吸过程中，卵母细胞的不同结构可能会丢失或损坏。部分原因是 ICSI 中使用的微型工具不是专门为辅助生殖设计的，而是为其他医学生物学学科设计的。鉴于上述注意事项，本研究的目的是将 ICSI 与传统卵母细胞保存移液器和无抽吸卵母细胞保存移液器 (PiWA) 的结果进行比较。该研究总共包括 155 名患者和 1037 个卵母细胞。在每个 ICSI 周期中，一半卵母细胞使用传统的固定移液器进行显微注射，另一半使用 PiWA 进行显微注射。结果，PiWA 技术显着提高了受精率：88.12%（95%CI：84.62–90.92%）；手持移液器：73.33% (95%CI: 68.72–77.49%)。此外，与传统的固定移液器相比，它的胚胎退化率显着降低[PiWA：2.07％（95％CI：1.11-3.8％）；固定移液器：4.51%（95%CI：3.06–6.59%）]。妊娠率取决于所使用的保存技术，无论是单胚胎移植（n = 59；χ 2 = 4.608；P值 = 0.032）还是双胚胎移植（n = 156；χ 2 = 4.344；P值 = 0.037） ; PiWA 的妊娠率明显高于传统的抱持技术。根据目前的证据和目前的结果，改进应集中在通过最大限度地减少或避免误吸和细胞质解体来降低显微注射本身的侵袭性，正如 PiWA 所成功实现的那样。