Abstract

Soil salinity has been one of the significant barriers to improving rice production and quality. According to reports, Bacillus spp. can be utilized to boost plant development in saline soil, although the molecular mechanisms behind the interaction of microbes towards salt stress are not fully known. Variations in rice plant protein expression in response to salt stress and plant growth-promoting rhizobacteria (PGPR) inoculations were investigated using a proteomic method and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). Findings revealed that 54 salt-responsive proteins were identified by mass spectrometry analysis (LC–MS/MS) with the Bacillus spp. interaction, and the proteins were functionally classified as gene ontology. The initial study showed that all proteins were labeled by mass spectrometry analysis (LC–MS/MS) with Bacillus spp. interaction; the proteins were functionally classified into six groups. Approximately 18 identified proteins (up-regulated, 13; down-regulated, 5) were involved in the photosynthetic process. An increase in the expression of eight up-regulated and two down-regulated proteins in protein synthesis known as chaperones, such as the 60 kDa chaperonin, the 70 kDa heat shock protein BIP, and calreticulin, was involved in rice plant stress tolerance. Several proteins involved in protein metabolism and signaling pathways also experienced significant changes in their expression. The results revealed that phytohormones regulated the manifestation of various chaperones and protein abundance and that protein synthesis played a significant role in regulating salt stress. This study also described how chaperones regulate rice salt stress, their different subcellular localizations, and the activity of chaperones.

中文翻译：

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盐胁迫条件下接种促生根际细菌的水稻植株的生长和蛋白质响应

摘要

土壤盐分一直是提高水稻产量和品质的重大障碍之一。据报道，芽孢杆菌属。尽管微生物与盐胁迫相互作用背后的分子机制尚不完全清楚，但可用于促进盐渍土中的植物发育。使用蛋白质组学方法和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）研究了水稻植物蛋白表达响应盐胁迫和植物促生根际细菌（PGPR）接种的变化。研究结果显示，通过芽孢杆菌属的质谱分析 (LC-MS/MS) 鉴定出了 54 种盐响应蛋白。相互作用，并且蛋白质在功能上被分类为基因本体。初步研究表明，所有蛋白质均通过芽孢杆菌属的质谱分析 (LC-MS/MS) 进行标记。相互作用; 这些蛋白质在功能上分为六组。大约 18 种已鉴定的蛋白质（上调，13 种；下调，5 种）参与光合作用过程。蛋白质合成中被称为分子伴侣的八种上调蛋白和两种下调蛋白的表达增加，例如 60 kDa 伴侣蛋白、70 kDa 热休克蛋白 BIP 和钙网蛋白，与水稻植物的胁迫耐受性有关。参与蛋白质代谢和信号传导途径的几种蛋白质的表达也经历了显着变化。结果表明，植物激素调节各种伴侣的表现和蛋白质丰度，蛋白质合成在调节盐胁迫中发挥着重要作用。这项研究还描述了分子伴侣如何调节水稻盐胁迫、其不同的亚细胞定位以及分子伴侣的活性。