In this study, a novel adsorbent was prepared by modification of graphite with deep eutectic solvent based on camphor–thymol (by the mole ratio of 1:1) and was utilized as a separation phase for pipette tip micro-solid phase extraction of mitoxantrone from seawater, drug, urine and blood samples. After extraction, mitoxantrone content of samples was determined by spectrophotometry at 260 nm wavelength. Various variables which could affect the extraction, including pH, amount of extracting phase, volumes of sample and eluent, type of eluent, and number of the extraction/ejection cycles of solution and eluent, were evaluated and optimized by both one-variable-at-a-time and response surface methodology methods. Observations indicated that the highest extraction achieved when 200 mg of the adsorbent was employed for 100 µL of sample solution with 7 extraction cycles at pH 7.0. Analyte was desorbed from the adsorbent by 200 µL of methanol (as eluent) after 3 ejection cycles. The linear dynamic range of the technique was between 1 and 1000 µg L⁻¹, and the limit of detection and quantification were 0.2 µg L⁻¹ and 0.7 µg L⁻¹, respectively. Finally, the proposed extraction was successfully employed for the determination of mitoxantrone in various real samples with complicated matrices.

本研究以樟脑-百里酚（摩尔比为1:1）为基础，通过低共熔溶剂对石墨进行改性，制备了一种新型吸附剂，并用作吸头微固相萃取中米托蒽醌的分离相。海水、药物、尿液和血液样本。提取后，通过分光光度法在260 nm波长下测定样品的米托蒽醌含量。可以影响萃取的各种变量，包括 pH、萃取相量、样品和洗脱液的体积、洗脱液的类型以及溶液和洗脱液的萃取/喷射循环次数，均通过单变量和优化-a-time 和响应面方法。观察结果表明，当对 100 µL 样品溶液使用 200 mg 吸附剂并在 pH 7.0 下进行 7 个萃取循环时，可获得最高萃取率。3 个喷射循环后，分析物通过 200 µL 甲醇（作为洗脱液）从吸附剂上解吸。该技术的线性动态范围在1至1000 µg L ^-1之间，检测限和定量限分别为0.2 µg L ^-1和0.7 µg L ^-1。最后，所提出的提取方法成功用于各种复杂基质样品中米托蒽醌的测定。