Powdery mildew, a fungal pathogen, poses significant threats to various plant species, including economically important ones. Conventional treatment methods involving compound fungicides, while common, raise environmental and social health concerns due to their potential adverse effects. Recent years have witnessed the rise of omics technologies and the innovative CRISPR-Cas9 gene editing system, drawing considerable attention as potential tools for disease management. This review aims to provide a comprehensive understanding of how omics and CRISPR-Cas9 are employed to unravel the molecular mechanisms of powdery mildew and to develop effective disease control strategies. The discussion delves into the utilization of genomics and transcriptomics methodologies to elucidate the intricate interactions between the powdery mildew pathogen and its host plants. Additionally, it explores the potential of CRISPR-Cas9 technology for targeted gene editing to enhance plant resistance against powdery mildew. The review goes further to propose a practical strategy for implementing these methods in the management of powdery mildew, offering insights into the selection of target genes and refining CRISPR-Cas9 delivery mechanisms. Overall, the review underscores the significant promise of omics and CRISPR-Cas9 technologies in advancing our comprehension of powdery mildew pathogenesis and provides valuable guidance on their application for efficient disease management.

中文翻译：

---

组学和 CRISPR-Cas9 分子感知：白粉病管理的渐进式审查方法

白粉病是一种真菌病原体，对多种植物物种（包括经济上重要的植物物种）构成重大威胁。涉及复合杀菌剂的传统处理方法虽然很常见，但由于其潜在的不利影响而引起了环境和社会健康问题。近年来，组学技术和创新的 CRISPR-Cas9 基因编辑系统的兴起，作为疾病管理的潜在工具而受到广泛关注。本综述旨在全面了解如何利用组学和 CRISPR-Cas9 来揭示白粉病的分子机制并制定有效的疾病控制策略。讨论深入探讨了利用基因组学和转录组学方法来阐明白粉病病原体与其宿主植物之间复杂的相互作用。此外，它还探索了 CRISPR-Cas9 技术用于靶向基因编辑以增强植物对白粉病的抗性的潜力。该综述进一步提出了在白粉病管理中实施这些方法的实用策略，为目标基因的选择和完善 CRISPR-Cas9 传递机制提供了见解。总体而言，该综述强调了组学和 CRISPR-Cas9 技术在促进我们对白粉病发病机制的理解方面的重大前景，并为其在有效疾病管理中的应用提供了宝贵的指导。