Aim: To investigate the correlation between doublecortin and CaM kinase-like-1 (DCAMKL-1) protein expression, K-ras gene mutation, and their impact on patient prognosis in colorectal cancer (CRC).
Methods: Immunohistochemistry was used to detect the expression of DCAMKL-1 protein in 60 cases of colorectal adenoma, 82 cases of CRC (including 65 cases of lymph node metastasis) and paraffin-embedded paracancerous intestinal mucosal tissue. K-ras gene mutations in primary CRC lesions were detected using an amplification-refractory mutation system and fluorescent polymerase chain reaction. The relationship between DCAMKL-1 protein expression and K-ras gene mutations with the clinicopathological characteristics of patients with CRC was analyzed. Univariate Kaplan‒Meier survival analysis and multivariate Cox regression analysis were performed using follow-up data.
Results: The mutation rate of the K-ras gene in 82 cases of CRC was 48.8% (40/82). The positivity rate for the presence of DCAMKL-1 protein in CRC was 70.7% (58/82), significantly higher than that for colorectal adenomas (53.3%; 32/60) and paracancerous intestinal mucosa (0%; 0/82) (P< 0.05). The positive expression rate for the presence of DCAMKL-1 protein in 65 patients with lymph node metastasis was higher in the primary lesions (69.2%; 45/65) than in the lymph node metastases (52.3%; 34/65) (χ²=12.087, P=0.001). The K-ras gene mutation status was positively correlated with DCAMKL-1 protein expression (r=0.252, P=0.022).
Conclusion: In this study, a potential positive correlation between K-ras gene mutation and DCAMKL-1 protein expression was identified in CRC tissues. The assessment of K-ras gene mutation status and DCAMKL-1 protein expression holds promise for augmenting early diagnosis and prognosis evaluation in CRC. This approach may improve the overall prognosis and survival outcomes for CRC patients.

Keywords: colorectal cancer, DCAMKL-1 protein, K-ras gene, mutation


中文翻译：

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结直肠癌中DCAMKL-1蛋白表达与K-ras基因突变的相关性

目的:探讨结直肠癌 (CRC) 中双皮质素与钙调蛋白激酶样 1 (DCAMKL-1) 蛋白表达、K-ras 基因突变的相关性及其对患者预后的影响。
方法:采用免疫组化方法检测60例结直肠腺瘤、82例结直肠癌(含淋巴结转移65例)及石蜡包埋癌旁肠黏膜组织中DCAMKL-1蛋白的表达情况。使用扩增阻滞突变系统和荧光聚合酶链反应检测原发性结直肠癌病变中的 K-ras 基因突变。分析DCAMKL-1蛋白表达及K-ras基因突变与CRC患者临床病理特征的关系。使用随访数据进行单变量 Kaplan-Meier 生存分析和多变量 Cox 回归分析。
结果： 82例结直肠癌中K-ras基因突变率为48.8%（40/82）。结直肠癌中 DCAMKL-1 蛋白的阳性率为 70.7%（58/82），显着高于结直肠腺瘤（53.3%；32/60）和癌旁肠粘膜（0%；0/82）。P <0.05）。65例淋巴结转移患者中DCAMKL-1蛋白阳性表达率在原发病灶中（69.2%；45/65）高于淋巴结转移灶（52.3%；34/65）（χ ² =12.087，P =0.001）。K-ras基因突变状态与DCAMKL-1蛋白表达量呈正相关（r =0.252，P =0.022）。
结论：在本研究中，结直肠癌组织中 K-ras 基因突变与 DCAMKL-1 蛋白表达之间存在潜在的正相关关系。K-ras 基因突变状态和 DCAMKL-1 蛋白表达的评估有望增强 CRC 的早期诊断和预后评估。这种方法可以改善结直肠癌患者的总体预后和生存结果。

Keywords:结直肠癌, DCAMKL-1蛋白, K-ras基因, 突变