The operational conditions for applying solutions of a dye (Oil Red) dissolved in acetone and methanol to a chromatographic plate are described. These solvents are characterized by high elution power, but they are very good solvents of various substances, so they are often used for this purpose in laboratory practice. However, their unfavorable feature, due to their use as solvents of samples placed on a chromatographic plate, is their high elution strength, which contributes to an increase of the diameter of the starting spot. To eliminate or reduce this unfavorable effect, this research used operational factors such as reduced speed of applying the test solution to the chromatographic plate, increased temperature (40 and 55 °C), and reduced pressure by 0.15 bar relative to atmospheric pressure. All this accelerated the evaporation of the solvents and led to smaller application spots. Studies have shown that the above-mentioned operational factors performed using equipment available in a regular laboratory contribute to a significant reduction of the starting spot despite the use of sample solvents with high elution strength.

描述了将溶解在丙酮和甲醇中的染料（油红）溶液施加到色谱板上的操作条件。这些溶剂的特点是洗脱能力高，但它们是各种物质非常好的溶剂，因此在实验室实践中经常用于此目的。然而，由于它们用作放置在色谱板上的样品的溶剂，它们的不利特征是它们的高洗脱强度，这有助于增加起始点的直径。为了消除或减少这种不利影响，本研究采用了一些操作因素，例如降低将测试溶液施加到色谱板上的速度、升高温度（40和55℃）以及相对于大气压降低0.15巴的压力。所有这些都加速了溶剂的蒸发并导致了更小的应用点。研究表明，尽管使用具有高洗脱强度的样品溶剂，但使用常规实验室中可用的设备执行的上述操作因素有助于显着减少起始点。