In recent studies with silver nanoparticles, it has been reported that the use of nanoparticles in carrier drug systems increases tumor suppression and reduces drug-related side effects. At the same time, the combination of traditional medicine with nanotechnology provides the opportunity to develop new antimicrobial agents. The aim of this study was to determine the anticancer, antimicrobial activities and pro-apoptotic effects of silver nanoparticles (AgNPs), and carboplatin-loaded silver nanoparticles (AgNPs-Car). Characterization studies of the synthesized nanoparticles were carried out by DLS, EDX-STEM, and FTIR analysis. The antiproliferative and pro-apoptotic effects of these molecules were evaluated using XTT and Annexin V, respectively. MIC (Minimum Inhibitory Concentration) test was used to determine the antimicrobial activity. The anticancer activity of the AgNPs-Car was high in MCF-7 (human breast adenocarcinoma), A549 (human lung carcinoma), and C6 (brain glioma) cells. The cell group with the most effective selective cytotoxic activity was C6 cells. It was also shown that AgNPs-Car and AgNPs induced DNA fragmentation eventually increasing apoptosis of cells. The antimicrobial activity of AgNPs and AgNPs-Car was evaluated on Gram-positive and Gram-negative pathogenic microorganisms and yeast fungi. Among the nanomaterials that reached effective MIC values according to reference sources, AgNPs-Car achieved better results. As a result, AgNPs-Car was found to be very successful in targeting C6 glioma cells by facilitating cell entry of the drug. In addition, their anticancer activity on MCF-7 and A549 cells was high and their toxicity was low. Silver nanoparticles are preferred for creating a better drug carrier system because of their qualitative properties and effects. Therefore, it is an interesting field for research on targeting cancer cells and pathogenic microorganisms.

中文翻译：

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负载卡铂的银纳米颗粒的合成和表征作为潜在的抗菌和癌症治疗

在最近对银纳米粒子的研究中，据报道，在载体药物系统中使用纳米粒子可以增加肿瘤抑制并减少与药物相关的副作用。同时，传统医学与纳米技术的结合为开发新型抗菌药物提供了机会。本研究的目的是确定银纳米颗粒 (AgNPs) 和卡铂银纳米颗粒 (AgNPs-Car) 的抗癌、抗菌活性和促凋亡作用。通过 DLS、EDX-STEM 和 FTIR 分析对合成的纳米颗粒进行表征研究。分别使用 XTT 和膜联蛋白 V 评估这些分子的抗增殖和促凋亡作用。MIC（最低抑菌浓度）测试用于测定抗菌活性。AgNPs-Car 在 MCF-7（人乳腺癌）、A549（人肺癌）和 C6（脑胶质瘤）细胞中具有较高的抗癌活性。具有最有效的选择性细胞毒活性的细胞群是C6细胞。还表明 AgNPs-Car 和 AgNPs 诱导 DNA 断裂，最终增加细胞凋亡。AgNPs 和 AgNPs-Car 对革兰氏阳性和革兰氏阴性病原微生物以及酵母真菌的抗菌活性进行了评估。根据参考来源，在达到有效 MIC 值的纳米材料中，AgNPs-Car 取得了更好的结果。结果发现，AgNPs-Car 通过促进药物进入细胞，非常成功地靶向 C6 神经胶质瘤细胞。此外，它们对MCF-7和A549细胞的抗癌活性高，毒性低。银纳米粒子因其质量特性和效果而成为创建更好的药物载体系统的首选。因此，针对癌细胞和病原微生物的研究是一个有趣的领域。