Tea (Camellia sinensis (L.) O. Kuntze.) is an economically important crop extensively grown for harvestable shoots in India. In the present study, 40 tea leaf samples (new leaves/buds) showing brown to black water-soaked expanding lesions collected from different commercial tea gardens of North Bengal and Assam, India, in different seasons were analyzed for the associated factor involved in the disease. A total of 40 isolates of bacteria with similar colony appearance on agar plates were isolated from symptomatic leaves/buds. Among the 40 isolates, six representative isolates from different tea gardens including three each from North Bengal (BBN01, BBP01, and BBJ01) and Assam (BBH01, BBD01, and BBA01) were characterized for gram reactions, biochemical tests, and by sequencing the 16S rRNA gene. All the isolates were gram negative, oxidase and catalase positive, and aerobically produced acid from glucose. BLASTn analysis of the obtained sequences revealed that all six isolates were Acidovorax avenae subsp. avenae, sharing a similarity greater than 99% with A. avenae subsp. avenae sequences from the GenBank database. Phylogenetic study also displayed the closest resemblance of these isolates to A. avenae subsp. avenae based on maximum likelihood. Further, six A. avenae subsp. avenae isolates were pathogenic on a tea cultivar TV23 in repeated experiments. Koch's postulates were verified by re-isolating the bacterial pathogen from the inoculated plants showing blight symptoms. In conclusion, this study confirms that A. avenae subsp. avenae is the bacterial pathogen responsible for causing symptoms of blight in tea leaves/buds. As far as we know, this is the first record of A. avenae subsp. avenae on Indian tea plants.

茶（Camellia sinensis (L.) O. Kuntze.）是一种重要的经济作物，在印度广泛种植用于收获芽。在本研究中，从印度北孟加拉邦和阿萨姆邦不同商业茶园不同季节采集的 40 个茶叶样品（新叶/芽）显示棕色至黑色的水浸扩展病斑，分析了影响茶病发生的相关因素。疾病。从有症状的叶子/芽中分离出总共 40 个在琼脂平板上具有相似菌落外观的细菌分离株。在 40 个分离株中，对来自不同茶园的 6 个代表性分离株进行了革兰氏反应、生化测试和 16S 测序，其中包括来自北孟加拉邦（BBN01、BBP01 和 BBJ01）和阿萨姆邦（BBH01、BBD01 和 BBA01）的各 3 个分离株。 rRNA基因。所有分离株均为革兰氏阴性、氧化酶和过氧化氢酶阳性，并且在需氧条件下从葡萄糖产生酸。对获得的序列进行 BLASTn 分析表明，所有 6 个分离株均为Acidovorax avenae subsp.。avenae，与A. avenae subsp.相似度大于 99% 。来自 GenBank 数据库的燕麦序列。系统发育研究还显示这些分离株与燕麦燕麦亚种(A. avenae subsp. ) 最相似。基于最大似然的大道。此外，六个燕麦亚种。在重复实验中，燕麦分离株对茶树品种 TV23 具有致病性。通过从表现出枯萎病症状的接种植物中重新分离细菌病原体，验证了科赫的假设。总之，本研究证实了A. avenae subsp。燕麦是引起茶叶/芽枯萎病症状的细菌病原体。据我们所知，这是A. avenae subsp.的第一个记录。印度茶树上的燕麦。