当前位置: X-MOL 学术Fish. Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Evaluating immobilisation thresholds and suitability of conductive glove electrodes for largemouth bass electroanaesthesia
Fisheries Research ( IF 2.4 ) Pub Date : 2024-01-04 , DOI: 10.1016/j.fishres.2023.106931
Connor H. Reid , Albana I. Berberi , Kara M. Scott , Sam J. Woods , Jonathan D. Midwood , Steven J. Cooke

Although electroanaesthesia (immobilising fish with weak currents and direct electrode contact for short-term handling and surgeries) is becoming more common, detailed data on electroanaesthesia immobilisation thresholds and potential morphological predictors of such thresholds remain sparse. We administered electroanaesthesia to largemouth bass (Micropterus nigricans) over a large range of body sizes using conductive mesh gloves connected to a power supply with fine-scale current control and real-time output display, noting current strengths at which tetany occurred. We also investigated whether a range of morphological indices were correlated with the currents required to induce tetany as a proxy for electroanaesthesia. Larger fish required stronger currents before tetany was observed, and larger fish were also more likely to fail to reach tetany before the maximum output on the apparatus (30 V) was reached, a finding that was slightly offset by increasing condition factor. Body length was a suitable predictor of the current required to induce tetany; no other morphometric indices examined were superior to length in this regard. However, there was very high variability in current strengths required to induce electroanaesthesia, likely attributable to consistency challenges associated with the use of conductive mesh gloves as electrodes (e.g., location of contact, wear-and-tear on gloves). We explore the implications of our results for applications of electroanaesthesia in the field, and make recommendations for the development and implementation of novel technologies and best practices when using electroanaesthesia on fish.



中文翻译:

评估大口黑鲈电麻醉的固定阈值和导电手套电极的适用性

尽管电麻醉(用弱电流和直接电极接触来固定鱼以进行短期处理和手术)变得越来越普遍,但有关电麻醉固定阈值和此类阈值的潜在形态学预测因子的详细数据仍然很少。我们使用连接到具有精细电流控制和实时输出显示的电源的导电网状手套,对各种体型的大口黑鲈( Micropterus nigricans )进行电麻醉,记录手足抽搐发生时的电流强度。我们还研究了一系列形态学指标是否与诱导手足抽搐所需的电流相关,作为电麻醉的代表。较大的鱼在观察到手足抽搐之前需要更强的电流,并且较大的鱼在达到设备的最大输出(30V)之前也更有可能无法达到手足抽搐,这一发现被增加的条件因素稍微抵消了。体长是诱发手足抽搐所需电流的合适预测因子。在这方面,没有其他检查的形态测量指标优于长度。然而,诱导电麻醉所需的电流强度存在非常高的可变性,这可能归因于与使用导电网手套作为电极相关的一致性挑战(例如,接触位置、手套的磨损)。我们探讨了我们的结果对电麻醉在该领域应用的影响,并为在鱼类上使用电麻醉时开发和实施新技术和最佳实践提出了建议。

更新日期:2024-01-06
down
wechat
bug