Bioactive compounds from the wood-decay fungus Xylaria cf. longipes SWUF08-81, cultivated in three different culture media (GM, YM and PDB), were isolated. Their structures and stereochemistry were deduced from spectroscopic and MS data analysis, together with quantum chemical calculations of ¹³C NMR chemical shifts and electronic circular dichroism (ECD) spectra. Five undescribed polyketides including dibenzofuran (1), mellein (2), dihydroisocoumarin (15), and two pyrans (16, 17), together with twenty-three compounds were determined. Compounds 18 and 20 were significantly toxic against cancer cell lines (HCT116, HT29, MCF-7 and HeLa) based on the MTT assay. Quantification by HPLC showed that 18 was produced three-fold higher in the broth of PDB than YM. These studies showed that the production of different compounds were primarily dependent on nutrition sources and it has given a starting point for the growth optimization conditions for the scaling up of bioactive compounds production.

Graphical Abstract

中文翻译：

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来自真菌 Xylaria 的抗增殖聚酮化合物 cf. 不同培养基中的长足 SWUF08-81

来自腐木真菌Xylaria的生物活性化合物cf. 分离了在三种不同培养基（GM、YM 和 PDB）中培养的长足虫SWUF08-81。它们的结构和立体化学是通过光谱和 MS 数据分析以及¹³ C NMR 化学位移和电子圆二色性 (ECD) 光谱的量子化学计算推导出来的。测定了五种未描述的聚酮化合物，包括二苯并呋喃 ( 1 )、mellein ( 2 )、二氢异香豆素 ( 15 ) 和两种吡喃 ( 16、17 )，以及 23 种化合物。根据 MTT 测定，化合物18和20对癌细胞系（HCT116、HT29、MCF-7 和 HeLa）具有显着毒性。HPLC 定量表明， PDB 肉汤中18 的产量是 YM 肉汤的三倍。这些研究表明，不同化合物的生产主要取决于营养源，并为扩大生物活性化合物生产的生长优化条件提供了起点。

图形概要