Abstract

The PBAF chromatin remodeling complex of the SWI/SNF family plays a critical role in the regulation of gene expression during tissue differentiation and organism development. The subunits of the PBAF complex have domains responsible for binding to N-terminal histone sequences. It determines the specificity of binding of the complex to chromatin. PHF10, a specific subunit of the PBAF complex, contains a DPF domain, which is a unique chromatin interaction domain. A PHF10 isoform that lacks the DPF domain is also present in vertebrate cells. This work shows that during neuronal and muscle differentiation of human and mouse cells, the expression of PHF10 isoforms changes: the form that does not have DPF replaces the form in which it is present. Replacement of PHF10 isoforms in the PBAF complex may affect its selectivity in the regulation of genes in differentiating cells.

中文翻译：

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哺乳动物细胞的神经元和肌肉分化伴随着 PHF10 亚型表达的变化

摘要

SWI/SNF 家族的 PBAF 染色质重塑复合物在组织分化和生物体发育过程中的基因表达调节中发挥着关键作用。PBAF 复合体的亚基具有负责结合 N 端组蛋白序列的结构域。它决定了复合物与染色质结合的特异性。PHF10 是 PBAF 复合物的一个特定亚基，包含一个 DPF 结构域，这是一个独特的染色质相互作用结构域。缺乏 DPF 结构域的 PHF10 亚型也存在于脊椎动物细胞中。这项工作表明，在人和小鼠细胞的神经元和肌肉分化过程中，PHF10 同工型的表达发生变化：不具有 DPF 的形式取代了其存在的形式。PBAF 复合物中 PHF10 亚型的替换可能会影响其在分化细胞中基因调节的选择性。