Two sorghum [Sorghum bicolor (L.) Moench] backcross-nested association mapping (BC-NAM) populations (Reg. no. MP-5, NSL 546724 MAP) have been developed, composed of 30 families totaling 2189 BC₁F₄ lines using an elite maintainer (B) or restorer (R) line as the recurrent parent. The families were derived from unadapted founder lines that were backcrossed to BTx623 and/or RTx436 based on the fertility reaction score and phylogenetic analysis of the founder lines. These unadapted founder lines were selected based on agronomic fitness, breeder desirability, and genetic diversity and represent a range of races and working groups. The 30 families were developed from 27 founder parents—three of the founder parents were backcrossed to both recurrent parents to provide a genetic relationship across all 30 families. Each family consists of 45–100 BC₁F₄ lines selected for standard agronomic fitness, and each population was genotyped using genotype-by-sequencing. To identify families that possess (or segregate for) traits of interest, 13 lines from each population were randomly selected and phenotyped for a range of agronomic and stress tolerance traits. These BC-NAM populations, which are adapted to US production regions and possess novel genetic diversity, have application in genetic research as a mapping resource for traits of interest and for sorghum breeding programs to diversify R and B line parental heterotic pools.

中文翻译：

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在 BTx623 或 RTx436 背景下注册高粱回交嵌套关联作图 (BC-NAM) 家族

已开发出两个高粱 [ Sorghum bicolor (L.) Moench] 回交巢式关联作图 (BC-NAM) 群体（注册号 MP-5，NSL 546724 MAP），由 30 个科组成，总计 2189 个 BC ₁ F ₄品系使用精英维持系（B）或恢复系（R）作为轮回亲本。这些家族源自未适应的创始品系，这些创始品系根据创始品系的育性反应评分和系统发育分析与 BTx623 和/或 RTx436 回交。这些未适应的创始品系是根据农艺适应性、育种意愿和遗传多样性进行选择的，代表了一系列种族和工作群体。这 30 个家族由 27 个创始亲本发展而来，其中三个创始亲本与两个轮回亲本回交，以在所有 30 个家族之间提供遗传关系。_{每个科由 45–100 个 BC 1} F ₄品系组成，这些品系根据标准农艺适应性进行选择，并且每个群体均使用基因型测序进行基因分型。为了识别拥有（或分离）感兴趣性状的家族，从每个群体中随机选择 13 个品系，并针对一系列农艺和胁迫耐受性状进行表型分析。这些 BC-NAM 群体适应美国生产地区并具有新颖的遗传多样性，可作为感兴趣性状的绘图资源和高粱育种计划应用于遗传研究，以使 R 和 B 系亲本杂种优势库多样化。