Bacterial cellulose (BC) is an extremely pure and highly valuable biomaterial. However, its production cost poses a challenge for large-scale manufacturing. This study explores a cost-effective approach by co-cultivating lactic acid bacteria with cellulose-synthesizing bacteria. Four BC-producing isolates from spoiled fruits and four lactic acid bacteria from fermented buttermilk were isolated and characterized. Growth studies demonstrated successful lactic acid bacteria cultivation in HS media. Co-cultivation of cellulose-synthesizing bacteria and lactic acid bacteria showed enhanced BC production, with a twofold increase in dry weight (0.35 g/150 ml) compared to the monoculture of cellulose-synthesizing bacteria (0.15 g/150 ml). Scanning electron microscopy revealed improved BC microfibril quality in co-culture. Reversed-phase HPLC confirmed higher lactic acid concentrations in co-culture. 16S rRNA sequence analysis revealed that lactic acid bacteria had a 100% match with Lactococcus lactis. These findings highlight the potential of co-cultivation for cost-effective BC production and lactic acid yield, offering a sustainable approach to biomaterial production.

细菌纤维素（BC）是一种极其纯净且极具价值的生物材料。然而，其生产成本给大规模生产带来了挑战。本研究探索了一种经济有效的方法，即共培养乳酸菌与纤维素合成细菌。分离并鉴定了四种产自腐败水果的 BC 菌株和四种来自发酵酪乳的乳酸菌。生长研究表明乳酸菌在 ​​HS 培养基中成功培养。纤维素合成细菌和乳酸菌的共培养显示出BC产量增强，与纤维素合成细菌单一培养（0.15 g/150 ml）相比，干重（0.35 g/150 ml）增加了一倍。扫描电子显微镜显示共培养中 BC 微纤维质量得到改善。反相 HPLC 证实共培养物中乳酸浓度较高。16S rRNA序列分析显示，乳酸菌与乳酸乳球菌100%匹配。这些发现凸显了共培养在具有成本效益的BC生产和乳酸产量方面的潜力，为生物材料生产提供了一种可持续的方法。