The use of fresh amniotic membrane (AM) is not a viable option, as it has many disadvantages. Preserving the AM reduces the risk of cross-infection and maintains its effectiveness for a long time. In order to maximize the therapeutic effects of the AM, the basic need is to preserve its vitality and the bioactive molecules it contains. However, the effect of preservation procedures on cell viability and growth factors is a still matter of debate. Optimum preservation method is expected to be cost-effective, easily-accessible, and most importantly, to preserve the effectiveness of the tissue for the longest time. However, each preservation technique has its advantages and disadvantages over the other, and each one compromises the vitality and bioactive molecules of the tissue to some extent. Therefore, the best method of preservation is still controversial, and the question of ‘how to preserve the AM best?’ has not yet been definitively answered.

使用新鲜羊膜 (AM) 并不是一个可行的选择，因为它有很多缺点。保存AM可降低交叉感染的风险并长期保持其有效性。为了最大限度地发挥 AM 的治疗效果，基本需要是保持其活力及其所含的生物活性分子。然而，保存程序对细胞活力和生长因子的影响仍然存在争议。最佳的保存方法预计具有成本效益、易于获得，最重要的是能够最长地保存组织的有效性。然而，每种保存技术都有其优点和缺点，并且每种技术都在一定程度上损害了组织的活力和生物活性分子。因此，最佳的保存方法仍然存在争议，“如何最好地保存AM？”的问题。尚未得到明确答复。