A novel detection method, known as RGB-tracking, was utilized to quantify the relative color changes in reactants/products during the dyeing of a solution of indigo as a model compound. The RGB-tracking system consists of a digital camera, a light source, a data acquisition device, a LabVIEW program, and its built-in function (Machine Vision). For the reduction of indigo, two types of reagents were employed: sodium dithionite (Na₂S₂O₄) for the chemical redox process and yeast (Saccharomyces cerevisiae) for the biological fermentation process. As a result, in the case of chemical redox during the reduction of the indigo solution, the progress of the reaction can be described by a color change, that is, the curves of R- and G-pixels, respectively. When the curve for the G-pixels began to increase, this indicated that leuco-indigo is being formed. A dramatic increase in the R-curve indicates that the amount of leuco-indigo has exceeded than that of indigo itself and that the reaction will gradually reach equilibrium. In contrast to this, additional complex behavior was observed in the case of biological fermentation. When the RGB-tracking curves for the R- and G-pixels reach their maximum values, this indicates that the concentration of leuco-indigo was significantly higher than that of indigo itself. The flattening of the RGB-tracking curves indicates that the reaction has reached equilibrium.

中文翻译：

---

使用 LabVIEW 和机器视觉实时分析和跟踪靛蓝溶液的还原情况

采用一种称为 RGB 跟踪的新颖检测方法来量化靛蓝溶液作为模型化合物的染色过程中反应物/产物的相对颜色变化。RGB跟踪系统由数码相机、光源、数据采集设备、LabVIEW程序及其内置函数（机器视觉）组成。为了还原靛蓝，使用了两种类型的试剂：用于化学氧化还原过程的连二亚硫酸钠（Na ₂ S ₂ O ₄ ）和用于生物发酵过程的酵母（酿酒酵母）。因此，在靛蓝溶液还原过程中发生化学氧化还原的情况下，反应的进程可以通过颜色变化来描述，即分别通过R像素和G像素的曲线来描述。当 G 像素的曲线开始增加时，这表明隐色靛蓝正在形成。R曲线的急剧增加表明无色靛蓝的量已经超过靛蓝本身的量并且反应将逐渐达到平衡。与此相反，在生物发酵的情况下观察到额外的复杂行为。当 R 和 G 像素的 RGB 跟踪曲线达到最大值时，这表明无色靛蓝的浓度明显高于靛蓝本身的浓度。RGB 跟踪曲线变平表明反应已达到平衡。