Oidium heveae HN1106, a powdery mildew (PM) that infects rubber trees, has been found to trigger disease resistance in Arabidopsis thaliana through ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-, PHYTOALEXIN DEFICIENT 4 (PAD4)- and salicylic acid (SA)-mediated signalling pathways. In this study, a typical TOLL-INTERLEUKIN 1 RECEPTOR, NUCLEOTIDE-BINDING, LEUCINE-RICH REPEAT (TIR-NB-LRR)-encoding gene, WHITE RUST RESISTANCE 4 (WRR4B), was identified to be required for the resistance against O. heveae in Arabidopsis. The expression of WRR4B was upregulated by O. heveae inoculation, and WRR4B positively regulated the expression of genes involved in SA biosynthesis, such as EDS1, PAD4, ICS1 (ISOCHORISMATE SYNTHASE 1), SARD1 (SYSTEMIC-ACQUIRED RESISTANCE DEFICIENT 1) and CBP60g (CALMODULIN-BINDING PROTEIN 60 G). Furthermore, WRR4B triggered self-amplification, suggesting that WRR4B mediated plant resistance through taking part in the SA-based positive feedback loop. In addition, WRR4B induced an EDS1-dependent hypersensitive response in Nicotiana benthamiana and contributed to disease resistance against three other PM species: Podosphaera xanthii, Erysiphe quercicola and Erysiphe neolycopersici, indicating that WRR4B is a broad-spectrum disease resistance gene against PMs.

中文翻译：

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WRR4B 有助于拟南芥对白粉病的广谱抗病性

Oidium heveae HN1106 是一种感染橡胶树的白粉病 (PM)，已被发现通过增强疾病易感性 1 (EDS1)、植物抗毒素缺陷 4 (PAD4) 和水杨酸 (SA) 介导，在拟南芥中引发抗病性信号通路。在这项研究中，一个典型的TOLL-白介素 1 受体、核苷酸结合、富含亮氨酸重复序列( TIR-NB-LRR ) 编码基因，即白锈病抗性 4 ( WRR4B )，被确定为抗O.拟南芥中的橡胶树。接种橡胶树后WRR4B的表达上调，并且WRR4B正向调节参与SA生物合成的基因的表达，例如EDS1、PAD4、ICS1（ISOCHORISMATE SYNTHASE 1）、SARD1（SYSTEMIC-ACQUIRED RESISTANCE DEFICIENT 1）和CBP60g（钙调蛋白结合蛋白 60 G )。此外，WRR4B 触发自我放大，表明 WRR4B 通过参与基于 SA 的正反馈循环介导植物抗性。此外，WRR4B在本塞姆氏烟草中诱导了EDS1依赖性超敏反应，并有助于对其他三种 PM 物种的抗病性：Podosphaera xanthii、Erysiphe quercicola和Erysiphe neolycopersici，表明WRR4B是针对 PM 的广谱抗病基因。