Blood is the most commonly used body fluid for obtaining and studying extracellular vesicles (EVs). While blood is a standard choice for clinical analysis, using blood as a source of EVs introduces multiple layers of complexity. At the Blood Extracellular Vesicle Workshop organized by the International Society for Extracellular Vesicles in Helsinki (2022), it became evident that beginner researchers lack trustworthy information on how to initiate their research and avoid common pitfalls. This educational guide explains the composition and frequently used terminology of blood, provides guidelines for blood collection, and the preparation of plasma and serum. It also introduces the basic principles of isolating and detecting blood EVs while considering blood-related factors. The goal of this guide is to assist beginners by offering a concise and evidence-based introduction to the current knowledge and available resources to study blood EVs.

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研究人血浆和血清中细胞外囊泡的初学者指南

血液是获取和研究细胞外囊泡 (EV) 最常用的体液。虽然血液是临床分析的标准选择，但使用血液作为 EV 来源会带来多层复杂性。在国际细胞外囊泡学会在赫尔辛基组织的血液细胞外囊泡研讨会（2022 年）上，很明显，初学者研究人员缺乏关于如何开始研究和避免常见陷阱的可靠信息。本教育指南解释了血液的成分和常用术语，提供了血液采集以及血浆和血清的制备指南。还介绍了在考虑血液相关因素的情况下分离和检测血液 EV 的基本原理。本指南的目的是通过对研究血液 EV 的当前知识和可用资源提供简明且基于证据的介绍来帮助初学者。