Cold plasma finds considerable interest in biodecontamination. A major issue is to elucidate the pathways of plasma–bacteria interaction. The present work aims at studying inactivation mechanisms for planktonic bacteria Escherichia coli and Staphylococcus aureus induced by cold plasma generated by a transient spark discharge. Changes in bacterial viability, metabolic activity, membrane integrity, intracellular reactive oxygen species level, and cell morphology reveal different patterns of cellular damage of the bacteria. Our results emphasize the importance of cell membrane integrity and maintenance of intracellular redox balatnce to resist plasma treatment. The physicochemical properties of the plasma-treated liquid (PTL) are monitored. Acidification and accumulation of various reactive species including •OH, H₂O₂, ONOOH, and NO₃⁻ in PTL play crucial roles in bacterial inactivation.

中文翻译：

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液体中瞬时火花放电对大肠杆菌和金黄色葡萄球菌的灭活途径

冷等离子体在生物净化方面引起了极大的兴趣。一个主要问题是阐明血浆-细菌相互作用的途径。目前的工作旨在研究 瞬态火花放电产生的冷等离子体诱导浮游细菌大肠杆菌和金黄色葡萄球菌的灭活机制。细菌活力、代谢活性、膜完整性、细胞内活性氧水平和细胞形态的变化揭示了细菌细胞损伤的不同模式。我们的结果强调了细胞膜完整性和维持细胞内氧化还原平衡以抵抗等离子体处理的重要性。监测等离子体处理液体 (PTL) 的物理化学性质。_{PTL 中各种活性物质（包括•OH、H 2} O ₂、ONOOH 和NO ₃ ^-）的酸化和积累在细菌灭活中发挥着至关重要的作用。