Aliarcobacter spp. have been isolated from numerous food products at retail and from animal carcasses and feces at slaughter. The objectives of this study were as follows: (i) to isolate Aliarcobacter species from different slaughterhouses’ samples and (ii) to detect genetic diversity, antibiotic resistance, biofilm ability, and putative virulence gene profiles of the isolates. A molecular investigation of antibiotic resistance and virulence factors was also conducted using polymerase chain reaction (PCR). Among 150 samples, a total of 22 (14.6%) Aliarcobacter spp. isolates were obtained, with varying levels of antibiotic resistance observed. The genes tetO, tetW, and gyrA were detected in 0%, 31.8%, and 27.2% of the isolates, respectively. All isolates were resistant to ampicillin, rifampin, and erythromycin, while tetracycline was found to be the most effective antibiotic, with 81.8% of the isolates showing susceptibility to it. All isolates (100%) harbored more than one of the nine putative virulence genes tested, with 18.1% of isolates carrying more than three. Regarding biofilm formation, 7 (31.8%) and 4 (18.1%) isolates were found to form strong and moderate biofilms, respectively, while one (4.5%) isolate was classified as a weak biofilm producer. ERIC-PCR band patterns suggested that the isolated Aliarcobacter spp. from slaughterhouses had different sources of contamination. These findings highlight the potential risk posed by pathogenic and multidrug-resistant Aliarcobacter spp. in food and the need for control measures throughout the food chain to prevent the spread of these strains. The results indicate that foods of animal origin and cattle slaughterhouses are significant sources of antimicrobial resistant Aliarcobacter.

阿里科杆菌属 已从零售的众多食品以及屠宰的动物尸体和粪便中分离出来。本研究的目的如下：(i) 从不同屠宰场的样本中分离出 Aliarcobacter 物种，以及 (ii) 检测分离株的遗传多样性、抗生素抗性、生物膜能力和假定的毒力基因谱。还使用聚合酶链反应（PCR）对抗生素耐药性和毒力因子进行了分子研究。在 150 个样品中，共有 22 个（14.6%）Aliarcobacter spp。获得了分离株，并观察到不同程度的抗生素耐药性。分别在 0%、31.8% 和 27.2% 的分离株中检测到基因tetO、tetW和gyrA 。所有分离株均对氨苄西林、利福平和红霉素耐药，而四环素被发现是最有效的抗生素，81.8%的分离株对其敏感。所有分离株 (100%) 均携带超过 1 种测试的 9 种假定毒力基因，其中 18.1% 的分离株携带 3 种以上。关于生物膜形成，发现 7 个（31.8%）和 4 个（18.1%）分离株分别形成强和中度生物膜，而 1 个（4.5%）分离株被归类为弱生物膜产生者。ERIC-PCR 带模式表明分离的Aliarcobacter spp。来自屠宰场的食品有不同的污染源。这些发现强调了致病性和多重耐药性阿里科杆菌属所带来的潜在风险。食品中存在这种菌株，并且需要在整个食物链中采取控制措施以防止这些菌株的传播。结果表明，动物源性食品和牛屠宰场是抗微生物药物的重要来源。