Background: Radiation therapy (RT) can cause changes in peripheral blood immune cells. The relationship between the efficacy of radiation therapy for non-small cell lung cancer (NSCLC) and immune cell changes and the study of how mediastinal radiation dose parameters affect immune cell changes is still unclear. This study aims to analyze the relationship between immune cell changes induced by radiotherapy and the efficacy of NSCLC radiotherapy, as well as the relationship between radiotherapy dose parameters and immune cell changes.
Materials and Methods: We retrospectively analyzed the data of NSCLC patients receiving mediastinal radiation therapy from 2020 to 2022. Collect lymphocytes and circulating immune cells within one week before and after radiotherapy and collect the dose-volume parameters of the whole mediastinum in the patient’s RT planning system. Analyze the changes in lymphocytes and radiotherapy effects after radiotherapy, and explore the relationship between radiotherapy dose parameters and immune cell changes.
Results: A total of 72 patients were enrolled. Compared with before radiotherapy, the proportion of CD3+T cells, CD8+T cells, and CD8/Treg in peripheral blood significantly increased after radiotherapy (P< 0.05). The increase in CD8+T cells and CD8/Treg after radiotherapy was correlated with Objective response rate (ORR) (P< 0.05). Based on binary logistic univariate and multivariate regression analysis, an increase in CD8+T cells after radiotherapy is an independent predictor of objective tumor response after radiotherapy (OR=12.71, 95% CI=3.64– 44.64, P=0.01), and Volume of 200 cGy irradiation (V2) is an independent positive predictor of an increase in CD8+T lymphocyte ratio after radiotherapy (high group, OR=3.40, 95% CI=1.13– 10.36, P=0.03).
Conclusion: The increase in CD8+T cells after radiotherapy can positively predict the short-term efficacy of radiotherapy. Mediastinal low-dose radiation therapy can increase CD8+T cells, thereby improving the short-term efficacy of radiotherapy. These potentially related mechanisms are worth further verification and exploration.



中文翻译：

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纵隔低剂量放疗面积和CD8+T细胞与非小细胞肺癌放疗疗效的相关性

背景：放射治疗（RT）可引起外周血免疫细胞的变化。非小细胞肺癌（NSCLC）放射治疗的疗效与免疫细胞变化之间的关系以及纵隔放射剂量参数如何影响免疫细胞变化的研究仍不清楚。本研究旨在分析放疗引起的免疫细胞变化与NSCLC放疗疗效的关系，以及放疗剂量参数与免疫细胞变化的关系。
材料与方法：我们回顾性分析了2020年至2022年接受纵隔放射治疗的NSCLC患者的数据。收集放疗前后1周内的淋巴细胞和循环免疫细胞，并收集患者放疗计划中全纵隔的剂量体积参数。系统。分析放疗后淋巴细胞的变化及放疗效果，探讨放疗剂量参数与免疫细胞变化的关系。
结果：共有 72 名患者入组。与放疗前相比，放疗后外周血 CD3+T 细胞、CD8+T 细胞、CD8/Treg 比例均显着升高（P < 0.05）。放疗后CD8+T细胞和CD8/Treg的增加与客观缓解率（ORR）相关（P < 0.05）。基于二元 Logistic 单变量和多变量回归分析，放疗后 CD8+T 细胞的增加是放疗后客观肿瘤反应的独立预测因子（OR=12.71，95% CI=3.64-44.64，P =0.01），并且200 cGy 照射（V2）是放疗后 CD8+T 淋巴细胞比例增加的独立阳性预测因子（高组，OR=3.40，95% CI=1.13-10.36，P =0.03）。
结论：放疗后CD8+T细胞的增加可以积极预测放疗的短期疗效。纵隔低剂量放疗可以增加CD8+T细胞，从而提高放疗的短期疗效。这些潜在的相关机制值得进一步验证和探索。