Endometriosis, a very common disease in women, is characterized by endometrial structures outside the uterine cavity. The lack of a reliable noninvasive diagnostic test and the often nonspecific symptoms of this pathology are responsible for the delay in definitive diagnosis of this disease. Recently, through a proteomics approach, our research group has identified two potential diagnostic markers for endometriosis in serum (Zn-alpha2-glycoprotein and complement C3 precursor). In this article, we describe the experimental conditions of a simple ELISA for rapid quantification of these two biomarkers in the saliva of patients with endometriosis. Finally, preliminary experiments on a small cohort of patients and controls have confirmed the potential diagnostic value of this assay.

中文翻译：

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用于子宫内膜异位症体外诊断的两种生物标志物定量的原型唾液测定

子宫内膜异位症是女性非常常见的疾病，其特征是子宫内膜结构位于子宫腔之外。缺乏可靠的非侵入性诊断测试以及这种病理学通常非特异性的症状是导致这种疾病的明确诊断延迟的原因。最近，通过蛋白质组学方法，我们的研究小组在血清中鉴定出了两种潜在的子宫内膜异位症诊断标志物（Zn-α2-糖蛋白和补体C3前体）。在本文中，我们描述了用于快速定量子宫内膜异位症患者唾液中这两种生物标志物的简单 ELISA 的实验条件。最后，对一小群患者和对照进行的初步实验证实了该测定的潜在诊断价值。