Microfluidics has emerged as a promising approach for assessing cellular behavior in vitro, providing more physiologically relevant cell culture environments with dynamic flow and shear stresses. This study introduces the Universal Biomaterial-on-Chip (UBoC) device, which enables the evaluation of cell response on diverse biomaterial substrates in a 3D-printed microfluidic device. The UBoC platform offers mechanical stimulation of the cells and monitoring of their response on diverse biomaterials, enabling qualitative and quantitative in vitro analysis both on- and off-chip. Cell adhesion and proliferation were assessed to evaluate the biocompatibility of materials with different physical properties, while mechanical stimulation was performed to investigate shear-dependent calcium signaling in pre-osteoblasts. Moreover, the applicability of the UBoC platform in creating more complex in vitro models by culturing multiple cell types was demonstrated, establishing a dynamic multicellular environment to investigate cellular interfaces and their significance in biological processes. Overall, the UBoC presents an adaptable tool for in vitro evaluation of cellular behavior, offering opportunities for studying various biomaterials and cell interactions in microfluidic environments.

Graphical Abstract

中文翻译：

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通用片上生物材料：用于评估多种生物材料基质上的细胞反应的多功能平台

微流体已成为评估体外细胞行为的一种有前景的方法，提供具有动态流动和剪切应力的更具生理相关性的细胞培养环境。本研究介绍了通用生物片上生物材料 (UBoC) 设备，该设备能够评估 3D 打印微流体设备中不同生物材料基材上的细胞反应。UBoC 平台提供细胞机械刺激并监测其对不同生物材料的反应，从而实现片内和片外定性和定量体外分析。评估细胞粘附和增殖以评估具有不同物理特性的材料的生物相容性，同时进行机械刺激以研究前成骨细胞中剪切依赖性钙信号传导。此外，还证明了 UBoC 平台在通过培养多种细胞类型创建更复杂的体外模型方面的适用性，建立动态多细胞环境来研究细胞界面及其在生物过程中的重要性。总体而言，UBoC 提供了一种用于体外评估细胞行为的适应性工具，为研究微流体环境中的各种生物材料和细胞相互作用提供了机会。

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