The embryonic development of the pig comprises a long in utero pre- and peri-implantation development, which dramatically differs from mice and humans. During this peri-implantation period, a complex series of paracrine signals establishes an intimate dialogue between the embryo and the uterus. To better understand the biology of the pig blastocyst during this period, we generated a large dataset of single-cell RNAseq from early and hatched blastocysts, spheroid and ovoid conceptus and proteomic datasets from corresponding uterine fluids. Our results confirm the molecular specificity and functionality of the three main cell populations. We also discovered two previously unknown subpopulations of the trophectoderm, one characterised by the expression of , which could represent progenitor cells, and the other, expressing pro-apoptotic markers, which could correspond to the Rauber's layer. Our work provides new insights into the biology of these populations, their reciprocal functional interactions, and the molecular dialogue with the maternal uterine environment.

中文翻译：

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从单细胞转录组学和子宫液蛋白质组学推断猪囊胚的细胞规格和功能相互作用

猪的胚胎发育包括长期的子宫内植入前和植入周围发育，这与小鼠和人类显着不同。在这个围植入期，一系列复杂的旁分泌信号在胚胎和子宫之间建立了密切的对话。为了更好地了解这一时期猪囊胚的生物学特性，我们从早期和孵化的囊胚、球体和卵形概念以及相应子宫液的蛋白质组数据集生成了一个大型单细胞 RNAseq 数据集。我们的结果证实了三个主要细胞群的分子特异性和功能。我们还发现了两个以前未知的滋养外胚层亚群，一个以表达 为特征，可能代表祖细胞，另一个则表达促凋亡标记，可能对应于劳伯层。我们的工作为这些群体的生物学、它们相互的功能相互作用以及与母体子宫环境的分子对话提供了新的见解。