The current study aimed to investigate genomic instabilities in healthcare workers who may experience varying levels of radiation exposure through various radiological procedures. It also sought to determine if factors related to the work environment and dosimeter reading could effectively explain the observed genomic instabilities. Utilizing the cytokinesis-block micronucleus assay (CBMN) on peripheral blood lymphocytes, we assessed a spectrum of genomic aberrations, including nucleoplasmic bridge (NPB), nuclear budding (NBUD), micronucleus (MN) formation, and total DNA damage (TDD). The study uncovered a statistically significant increase in the occurrence of distinct DNA anomalies among radiology workers (with a significance level of P < 0.0001 for all measurements). Notably, parameters such as total working hours, average work duration, and time spent in projection radiography exhibited significant correlations with MN and TDD levels in these workers. The dosimeter readings demonstrated a positive correlation with the frequency of NPB and NBUD, indicating a substantial association between radiation exposure and these two genomic anomalies. Our multivariable models identified the time spent in projection radiography as a promising parameter for explaining the overall genomic instability observed in these professionals. Thus, while dosimeters alone may not fully explain elevated total DNA damage, intrinsic work environment factors hold potential in indicating exposure levels for these individuals, providing a complementary approach to monitoring.

目前的研究旨在调查可能通过各种放射程序经历不同程度的辐射暴露的医护人员的基因组不稳定性。它还试图确定与工作环境和剂量计读数相关的因素是否可以有效解释观察到的基因组不稳定性。利用外周血淋巴细胞的胞质分裂阻断微核测定 (CBMN)，我们评估了一系列基因组畸变，包括核质桥 (NPB)、核出芽 (NBUD)、微核 (MN) 形成和总 DNA 损伤 (TDD)。该研究发现，放射科工作人员中明显 DNA 异常的发生率在统计上显着增加（所有测量的显着性水平 P < 0.0001）。值得注意的是，总工作时间、平均工作持续时间和投影射线照相花费的时间等参数与这些工人的 MN 和 TDD 水平显着相关。剂量计读数显示与 NPB 和 NBUD 的频率呈正相关，表明辐射暴露与这两种基因组异常之间存在显着关联。我们的多变量模型将投影射线照相所花费的时间确定为解释这些专业人员观察到的整体基因组不稳定性的一个有希望的参数。因此，虽然剂量计本身可能无法完全解释总 DNA 损伤升高的情况，但内在的工作环境因素有可能指示这些个体的暴露水平，从而提供一种补充监测方法。