Soft rot disease is one of the primary issues in the production of colored calla lilies, significantly limiting large-scale production. This research conducts an in-depth investigation of the class III peroxidase gene family in colored calla lily, identifying 80 genes, with their motifs and structural domains remaining conserved. Through phylogenetic tree analysis, these genes were classified into 9 distinct clades. Collinearity analysis and the distribution of genes suggest that the two main events of gene family evolution was whole-genome duplication (WGD) as well as tandem duplication. -acting element analysis showed that the genes could be directly modulated by essential hormones like jasmonic acid (JA) and salicylic acid (SA), highlighting their role in biotic stress responses. Transcriptome profiling revealed tissue-specific gene expressions and their essential function in responding to soft rot bacteria. Upon inoculation with , the leaves manifested a significant upregulation of genes expression, and there was a concurrent surge in JA and SA content. Treatments with methyl jasmonate (MeJA) and benzothiadiazole (BTH) induced gene expression and augmented the plant's disease resistance. This indicates the crucial role of genes in regulating reactive oxygen species in leaves. In summary, these findings emphasize the importance of genes in the defense mechanisms of colored calla lily.

中文翻译：

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彩色马蹄莲中 III 类过氧化物酶的全基因组鉴定和增强对软腐菌的抵抗力

软腐病是彩色马蹄莲生产中的主要问题之一，严重限制了大规模生产。本研究对彩色马蹄莲中的 III 类过氧化物酶基因家族进行了深入研究，鉴定出 80 个基因，其基序和结构域保持保守。通过系统发育树分析，这些基因被分为9个不同的分支。共线性分析和基因分布表明，基因家族进化的两个主要事件是全基因组复制（WGD）和串联复制。作用元件分析表明，这些基因可以直接受到茉莉酸（JA）和水杨酸（SA）等必需激素的调节，突出了它们在生物应激反应中的作用。转录组分析揭示了组织特异性基因表达及其在响应软腐病细菌中的基本功能。接种后，叶片表现出基因表达显着上调，并且 JA 和 SA 含量同时激增。用茉莉酸甲酯（MeJA）和苯并噻二唑（BTH）处理可诱导基因表达并增强植物的抗病性。这表明基因在调节叶子活性氧方面发挥着至关重要的作用。总之，这些发现强调了基因在彩色马蹄莲防御机制中的重要性。