Aberrant expression of microRNAs (miRNAs) has been shown to be linked to several crucial biological processes, including as carcinogenesis, metastasis, and progression. The advancement of innovative miRNA detection technologies can enhance the early detection of malignancies by merging with conventional diagnostic methods, such as ultrasound technology. Herein, we reported a simple, sensitive, and label-free miRNA detection method by integrating the proximity-catalytic hairpin assembly (proximity-CHA) and DNAzyme-assisted signal amplification. Compared with traditional CHA, in which the signal amplification efficiency is greatly limited by the concentration of hairpin probes, the proposed method possesses a greatly improved signal amplification efficiency. The target facilitated the non-enzymatic CHA-driven sequential formation of DNAzyme nanostructures, resulting in the effective DNAzyme-facilitated cleavage of a substrate modified with a fluorophore and quencher, leading to the production of an intensified fluorescence signal. The proximity-CHA-DNAzyme system possesses appealing analytical characteristics, making it highly promising for the analysis of many analytes in clinical research domains.

中文翻译：

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一种简单且无标记的基于邻近催化发夹反应的灵敏分析和 miRNA 分析方法

microRNA (miRNA) 的异常表达已被证明与几个重要的生物过程有关，包括癌发生、转移和进展。创新性 miRNA 检测技术的进步可以通过与超声技术等传统诊断方法相结合来增强恶性肿瘤的早期检测。在此，我们报道了一种简单、灵敏、无标记的 miRNA 检测方法，通过整合邻近催化发夹组装（proximity-CHA）和 DNAzyme 辅助信号放大。与信号放大效率受到发夹探针浓度极大限制的传统CHA相比，该方法的信号放大效率大大提高。该靶标促进了非酶 CHA 驱动的 DNAzyme 纳米结构的顺序形成，导致 DNAzyme 促进荧光团和猝灭剂修饰的底物的有效裂解，从而产生增强的荧光信号。Proximity-CHA-DNAzyme 系统具有吸引人的分析特性，使其在临床研究领域的许多分析物分析中极具前景。