Fagonia indica Burm.f. (1768) is a medicinally important plant showing diverse pharmaceutical benefits. It is renowned for its ability to biosynthesize several anticancer and anti-inflammatory metabolites. For the eco-friendly and sustainable synthesis of phytochemicals and plant biomass, a biotechnological technique, “elicitation,” is a highly effective method in various in vitro cultures. The present study includes using various concentrations of Fusarium oxysporum Schlecht. as an elicitor in callus cultures of Fagonia indica. The main goal was to achieve enhancement in biomass production and secondary metabolism. The findings demonstrated that maximum biomass production (FW: 167.42 ± 3.99 g per 100 mL; DW: 12.53 ± 1.04 g per 100 mL) was observed at 50 mg L⁻¹ of Fusarium oxysporum as compared to the control. Secondary metabolites showed immense production (phenolic content (9.68 ± 0.23 µg mg⁻¹); flavonoid content (2.814808 ± 0.11 µg mg⁻¹)) in callus cultures treated with 10 mg L⁻¹ of Fusarium oxysporum as compared with control. Moreover, the cultures possessed the highest antioxidant capacity, as determined by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS^•+) radical cation based assay and α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging assay, ((821.51 ± 3.20 µmol TEAC per mg DW of ABTS inhibition) (91% ± 1.45 of DPPH inhibition)) at 10 mg L⁻¹ concentration of Fusarium oxysporum, and the maximum ferric ion reducing activity (219.29 ± 2.36 µmol TEAC per mg DW) was noticed at 1.0 mg L⁻¹ concentration of F. oxysporum. Fagonia indica cultures also indicated the highest percent inhibition against cyclooxygenases (COX-1: 51.93% ± 1.74 and COX-2: 40.57% ± 1.99), lipoxygenase (15-LOX: 65.72% ± 1.44), and phospholipase A2 (sPLA2: 49.29% ± 1.75), when treated with different concentrations of F. oxysporum. HPLC analyses showed a significant accumulation of pharmacologically active components in the treated samples, with kaempferol (1245.56 mg g⁻¹) and myricetin (1139.63 mg g⁻¹) as the most accumulated compounds in the cultures with 10.0 mg L⁻¹ concentration of Fusarium in contrast to the control. These findings revealed that in callus cultures of F. indica, F. oxysporum could boost biomass accumulation and secondary metabolite production.

Fagonia indica Burm.f. (1768)是一种具有多种药用价值的重要药用植物。它以其生物合成多种抗癌和抗炎代谢物的能力而闻名。对于植物化学物质和植物生物质的生态友好和可持续合成，生物技术“诱导”是各种体外培养中的高效方法。本研究包括使用不同浓度的尖孢镰刀菌Schlecht。作为Fagonia indica愈伤组织培养物中的诱导子。主要目标是提高生物量生产和次生代谢。结果表明，与对照相比，在 50 mg L ^-1尖孢镰刀菌下观察到最大生物量产量（FW：167.42 ± 3.99 g/100 mL；DW：12.53 ± 1.04 g/100 mL） 。与对照相比，在用 10 mg L ^-1尖孢镰刀菌处理的愈伤组织培养物中，次生代谢物显示出巨大的产量（酚含量（9.68 ± 0.23 µg mg ^-1）；类黄酮含量（2.814808 ± 0.11 µg mg -1 ^）） 。此外，通过基于 2,2'-azino-bis(3-乙基苯并噻唑啉-6-磺酸) (ABTS ^•+ ) 自由基阳离子测定和 α, α-二苯基-β-苦基肼测定，培养物具有最高的抗氧化能力(DPPH) 自由基清除测定，((821.51 ± 3.20 µmol TEAC/mg DW ABTS 抑制) (DPPH 抑制的 91% ± 1.45)) 在 10 mg L ^-1浓度的尖孢镰刀菌下，以及最大铁离子还原活性(219.29 ± 2.36 µmol TEAC/mg DW) 在 1.0 mg L ^-1浓度的尖孢镰刀菌下观察到。Fagonia indica培养物还表明对环氧合酶（COX-1：51.93% ± 1.74 和 COX-2：40.57% ± 1.99）、脂氧合酶（15-LOX：65.72% ± 1.44）和磷脂酶 A2（sPLA2：49.29）的抑制百分比最高% ± 1.75)，用不同浓度的尖孢镰刀菌处理时。HPLC分析显示，处理后的样品中药理活性成分显着积累，其中山奈酚（1245.56 mg g ^-1）和杨梅素（1139.63 mg g ^{-1 ）是}镰刀菌浓度为10.0 mg L ^-1的培养物中积累最多的化合物。与对照相反。这些发现表明，在F. indica的愈伤组织培养物中，F. oxysporum可以促进生物量积累和次生代谢产物的产生。