Gancao Xiexin Decoction (GCXXD) is a traditional Chinese decoction that is often used in treating gastric ulcers. However, the substance basis and mechanism of action remain unclear. In this study, in vivo and in vitro components of GCXXD were analyzed by ultra-high-performance liquid chromatography coupled with quadrupole-orbitrap mass spectrometry. The compound Discover platform was used to ultimately enable rapid identification of compounds. Acquire X intelligent data acquisition technology software was innovatively adopted. In the process of collecting drug-containing plasma, all components detected in blank plasma samples were excluded to eliminate the interference and influence of endogenous components in plasma, making the analysis results more accurate and reliable. At the same time, the possibility of selecting precursor parent ions with low concentration levels within the chromatographic peak can be increased, improving the coverage and integrality of the detection of components in vivo. Also, the targeted network pharmacology strategy combined with molecular docking was established to explore the mechanism of GCXXD in treating gastric ulcers. As a result, 113 components were identified, 41 of which could enter the bloodstream and exert therapeutic effects in vivo. The main effective components are glycyrrhizic acid, 6-gingerol, jatrorrhizine, wogonin, palmatine, and liquiritigenin, main targets in vivo were related to ALB, IL6, and VEGF, which play an important role in anti-inflammatory and promoting angiogenesis. In summary, this study adopted a comprehensive analysis strategy to reveal the pharmacodynamic material basis and mechanism of GCXXD against gastric ulcers, providing a scientific basis for its clinical application.

中文翻译：

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基于超高效液相色谱联用四极轨道阱质谱的甘草泻心汤治疗胃溃疡的成分分析及网络药理学策略

甘草泻心汤（GCXXD）是一种传统中药汤剂，常用于治疗胃溃疡。然而，其物质基础和作用机制仍不清楚。在本研究中，通过超高效液相色谱结合四极杆轨道阱质谱分析了 GCXXD 的体内和体外成分。化合物发现平台用于最终实现化合物的快速识别。创新采用Acquire X智能数据采集技术软件。在采集含药血浆的过程中，将空白血浆样本中检测到的所有成分排除在外，消除了血浆中内源性成分的干扰和影响，使分析结果更加准确可靠。同时，可以增加选择色谱峰内低浓度母离子的可能性，提高体内成分检测的覆盖率和完整性。并建立靶向网络药理学策略结合分子对接，探讨GCXXD治疗胃溃疡的作用机制。结果，鉴定出113种成分，其中41种可以进入血液并在体内发挥治疗作用。主要有效成分为甘草酸、6-姜酚、药根碱、汉黄芩素、巴马汀、甘草素等，体内作用靶点主要为ALB、IL6、VEGF等，在抗炎、促进血管生成等方面发挥重要作用。综上所述，本研究采用综合分析策略，揭示GCXXD抗胃溃疡的药效物质基础和作用机制，为其临床应用提供科学依据。