Lymphedema, resulting from impaired lymphatic drainage, causes inflammation, fibrosis and tissue damage leading to symptoms such as limb swelling and restricted mobility. Despite various treatments under exploration, no standard effective therapy exists. Here a novel technique using the pyro-drive jet injection (PJI) was used to create artificial clefts between collagen fibers, which facilitated the removal of excess interstitial fluid. The PJI was used to deliver a mixture of lactated Ringer’s solution and air into the tail of animals with secondary skin edema. Edema levels were assessed using micro-CT scanning. Histopathological changes and neovascularization were evaluated on the injury-induced regenerative tissue. Regarding tissue remodeling, we focused on connective tissue growth factor (CTGF) and vascular endothelial growth factor (VEGF)-C. PJI markedly diminished soft tissue volume in the experimental lymphedema animals compared to the non-injected counterparts. The PJI groups exhibited a significantly reduced proportion of inflammatory granulation tissue and an enhanced density of lymphatic vessels and α-smooth muscle actin (αSMA)-positive small vessels in the fibrous granulation tissue compared to the controls. In addition, PJI curtailed the prevalence of CTGF- and VEGF-C-positive cells in regenerative tissue. In a lymphedema animal model, PJI notably ameliorated interstitial edema, promoted lymphatic vessel growth, and bolstered αSMA-positive capillaries in fibrous granulation tissue. PJI’s minimal tissue impact post-lymph node dissection indicates significant potential as an early, standard preventative measure. Easily applied in general clinics without requiring specialized training, it offers a cost-effective and highly versatile solution to the management of lymphedema.

淋巴引流受损导致淋巴水肿，引起炎症、纤维化和组织损伤，导致肢体肿胀和活动受限等症状。尽管正在探索各种治疗方法，但尚不存在标准的有效疗法。这里采用了一种利用热驱动喷射注射（PJI）的新技术在胶原纤维之间创建人工裂缝，这有助于去除多余的间质液。PJI 用于将乳酸林格氏溶液和空气的混合物输送到继发性皮肤水肿动物的尾部。使用显微 CT 扫描评估水肿水平。对损伤诱导的再生组织进行组织病理学变化和新血管形成的评估。关于组织重塑，我们重点关注结缔组织生长因子（CTGF）和血管内皮生长因子（VEGF）-C。与未注射的动物相比，PJI 显着减少了实验性淋巴水肿动物的软组织体积。与对照组相比，PJI 组的炎症肉芽组织比例显着减少，纤维肉芽组织中淋巴管和 α-平滑肌肌动蛋白 (αSMA) 阳性小血管的密度增加。此外，PJI 减少了再生组织中 CTGF 和 VEGF-C 阳性细胞的患病率。在淋巴水肿动物模型中，PJI 显着改善间质水肿，促进淋巴管生长，并增强纤维肉芽组织中的 αSMA 阳性毛细血管。PJI 在淋巴结清扫后对组织的影响极小，表明其作为早期标准预防措施的巨大潜力。它可以轻松应用于普通诊所，无需专门培训，为淋巴水肿的治疗提供了一种经济有效且高度通用的解决方案。