The Annexin 2 protein (ANXA2) encoded by the ANXA2 gene performs a number of biological functions that are primarily related to cellular transport. An impaired functional activity of the ANXA2 gene have been primarily detected in oncological diseases; however, we have previously shown an increased expression of the Anxa2 gene at the mRNA level in the early stages of neurodegeneration. In this regard, it is most interesting to study the effect of suppressed expression of the ANXA2 gene on nervous-system functioning in a Danio rerio model with the use of morpholino oligonucleotides (morpholino). The present study examined the effect of morpholino to the anxa2a gene on the development of Danio rerio embryo. The study was conducted with D. rerio line AB, embryos of which were injected with morpholino oligonucleotides to the anxa2a gene. The estimate of the effect of injected morpholino oligonucleotides on embryo development was examined by the changes in the phenotype on the second and fourth day post fertilization (dpf). An injection of experimental morpholino targeting the start-codon and 5'UTR region results in an increased percentage of deformations compared with the Co–In and Co–Mo–D groups at the fourth dpf. The deformations observed at the fourth dpf were evident in both control and experimental groups. In addition, we analyzed an earlier time point such as the second dpf. Phenotype changes at this time point were detected only in the experimental groups. A comparative analysis of data obtained from injected morpholino targeting start-codon and 5'UTR of the anxa2a gene evidence that both variants may be used for further research. In addition, we have shown that it is preferable to carry out analysis of expression at the second dpf. Apparently, the effects detected at the second dpf are specific, while the presence of a hindbrain ventricular deformity suggests that altered expression of the anxa2a gene may result in dysfunction of the nervous system.

由 ANXA2 基因编码的膜联蛋白 2 蛋白 ( ANXA2 )执行许多主要与细胞运输相关的生物学功能。ANXA2基因的功能活性受损主要在肿瘤疾病中被发现；然而，我们之前已经表明，在神经退行性变的早期阶段， Anxa2基因在 mRNA 水平上的表达增加。在这方面，最有趣的是使用吗啉代寡核苷酸（吗啉代）研究ANXA2基因表达抑制对斑马鱼模型中神经系统功能的影响。本研究探讨了吗啉代 anxa2a基因对斑马鱼胚胎发育的影响。这项研究是用斑马鱼品系 AB 进行的，该品系的胚胎被注射了吗啉寡核苷酸至anxa2a基因。通过受精后第二天和第四天（dpf）表型的变化来检查注射吗啉代寡核苷酸对胚胎发育的影响的估计。与 Co-In 和 Co-Mo-D 组相比，在第四个 dpf 时，针对起始密码子和 5'UTR 区域注射实验吗啉会导致变形百分比增加。在第四个 dpf 时观察到的变形在对照组和实验组中都很明显。此外，我们还分析了更早的时间点，例如第二个 dpf。仅在实验组中检测到该时间点的表型变化。对注射的吗啉靶向起始密码子和anxa2a基因 5'UTR 获得的数据进行比较分析，证明这两种变体均可用于进一步研究。此外，我们已经表明，最好在第二个 dpf 处进行表达分析。显然，在第二个 dpf 中检测到的影响是特定的，而后脑室畸形的存在表明anxa2a基因表达的改变可能会导致神经系统功能障碍。