Limb autotomy and regeneration represent distinctive responses of crustaceans to environmental stress. Glucose metabolism plays a pivotal role in energy generation for tissue development and regeneration across various species. However, the relationship between glucose metabolism and tissue regeneration in crustaceans remains elusive. Therefore, this study is aimed at analyzing the alterations of glucose metabolic profile during limb autotomy and regeneration in Eriocheir sinensis, while also evaluating the effects of carbohydrate supplementation on limb regeneration. The results demonstrated that limb autotomy triggered a metabolic profile adaption at the early stage of regeneration. Hemolymph glucose levels were elevated, and multiple glucose catabolic pathways were enhanced in the hepatopancreas. Additionally, glucose and ATP levels in the regenerative limb were upregulated, along with increased expression of glucose transporters. Furthermore, the gene expression and activity of enzymes involved in gluconeogenesis were repressed in the hepatopancreas. These findings indicate that limb regeneration triggers metabolic profile adaptations to meet the elevated energy requirements. Moreover, the study observed that supplementation with corn starch enhanced limb regeneration capacity by promoting wound healing and blastema growth. Interestingly, dietary carbohydrate addition influenced limb regeneration by stimulating gluconeogenesis rather than glycolysis in the regenerative limb. Thus, these results underscore the adaptation of glucose metabolism during limb autotomy and regeneration, highlighting its essential role in the limb regeneration process of E. sinensis.

肢体自切和再生代表了甲壳类动物对环境压力的独特反应。葡萄糖代谢在各个物种的组织发育和再生的能量产生中发挥着关键作用。然而，甲壳类动物的葡萄糖代谢和组织再生之间的关系仍然难以捉摸。因此，本研究旨在分析中华绒螯蟹肢体自切和再生过程中葡萄糖代谢谱的变化，同时评估碳水化合物补充对肢体再生的影响。结果表明，肢体自切在再生早期引发了代谢谱的适应。血淋巴葡萄糖水平升高，肝胰腺中多种葡萄糖分解代谢途径增强。此外，再生肢体中的葡萄糖和 ATP 水平上调，同时葡萄糖转运蛋白的表达增加。此外，肝胰腺中参与糖异生的酶的基因表达和活性受到抑制。这些发现表明，肢体再生会触发代谢特征的适应，以满足增加的能量需求。此外，研究发现补充玉米淀粉可以促进伤口愈合和芽基生长，从而增强肢体再生能力。有趣的是，膳食碳水化合物的添加通过刺激再生肢体中的糖异生而不是糖酵解来影响肢体再生。因此，这些结果强调了葡萄糖代谢在肢体自切和再生过程中的适应，突出了其在中华棘蜥肢体再生过程中的重要作用。