The unicellular green alga Oophila amblystomatis was named by Lambert in 1905 based upon its association with egg masses of the spotted salamander Ambystoma maculatum. We collected algal cells from Lambert's original egg capsule preparations that were contributed to Phycotheca Boreali-Americana (PBA) in 1905 and subjected them to DNA extraction and PCR with O. amblystomatis-specific 18S rRNA gene primers. DNA amplified from these preparations was cloned and nine clones were sequenced. Along with representative sequences from the Oophila clade and Chlorophyceae, a phylogenetic tree was inferred. Seven sequences clustered within the Oophila clade and two clustered with Chlamydomonas moewusii, which is included in a sister clade to Oophila. By sequencing algal material from the egg capsules of representative type material we can unambiguously characterize O. amblystomatis and define a monophyletic clade centered on this type material. Accordingly, we reject a recent proposal that this species be transferred to Chlorococcum.

中文翻译：

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Oophila amblystomatis（绿藻纲，衣藻目）的分类和命名

单细胞绿藻Oophila amblystomatis是兰伯特于 1905 年根据其与斑点蝾螈卵块的关系而命名的。我们从 Lambert 于 1905 年贡献给北美藻藻(PBA)的原始卵囊制剂中收集了藻细胞，并使用O. amblystomatis特异性 18S rRNA 基因引物对它们进行 DNA 提取和 PCR。对从这些制备物中扩增的 DNA 进行克隆并对 9 个克隆进行测序。连同Oophila分支和绿藻纲的代表性序列一起，推断出系统发育树。七个序列聚集在Oophila分支内，两个序列聚集在Chlamydomonas moewusii中，后者属于Oophila的姐妹分支。通过对代表性类型材料的卵囊中的藻类材料进行测序，我们可以明确地表征O. amblystomatis并定义以该类型材料为中心的单系进化枝。因此，我们拒绝最近将该物种转移到绿球藻的提议。