Among the methods for onsite detection of specific biomolecules, the use of retroreflective Janus particles (RJPs) is a simple and efficient method for biophotonic probes to detect biotarget molecules with spatioselectivity, which can make the RJPs align toward light sources after capturing the target biomolecules, enhancing retroreflection. Because RJP has two distinct regions with different properties, specific reagents can be easily functionalized through chemical reactions between the functional groups and biotarget molecules. The number of functional groups on RJP will be the critical parameter for efficiency of sensing ability. Here, clickable (azide) polymer brush is introduced on surface of RJP via different surface polymerization methods (grafting to and grafting from method). Azide groups allow for the easy modification of dibenzocyclooctyne-linked biomolecules onto silica particles through click chemistry. RJPs are prepared with different quantities of azide functional groups on their surfaces via solvent volatilization method and metal deposition. Using a retroreflective immunosensing system, sensitive detection of target biomolecules is performed.

中文翻译：

---

通过控制 Janus 粒子上的聚合物刷提高基于逆反射的生物传感器的灵敏度

在现场检测特定生物分子的方法中，利用逆反射Janus粒子（RJPs）是一种简单有效的生物光子探针检测具有空间选择性的生物目标分子的方法，它可以使RJPs在捕获目标生物分子后向光源对准，增强逆反射。由于 RJP 具有两个具有不同特性的不同区域，因此可以通过官能团和生物靶分子之间的化学反应轻松地对特定试剂进行功能化。 RJP 上的官能团数量将是传感能力效率的关键参数。在这里，通过不同的表面聚合方法（接枝到方法和接枝方法）在 RJP 表面引入可点击（叠氮化物）聚合物刷。叠氮基团允许通过点击化学将二苯并环辛炔连接的生物分子轻松修饰到二氧化硅颗粒上。通过溶剂挥发法和金属沉积法在其表面制备了不同数量的叠氮官能团的RJP。使用逆反射免疫传感系统，对目标生物分子进行灵敏检测。