In adult olfactory epithelium (OE), ATP plays a role in constant cell turnover and post-injury neuroregeneration. We previously demonstrated that constitutive and ATP-evoked ATP release are present in neonatal mouse OE and underlie continuous cell turn-over and post-injury neuroregeneration, and that activation of purinergic P2X7 receptors is involved in the evoked release. We hypothesized that both releases are present in adult mouse OE. To study the putative contribution of olfactory sensory neurons to ATP release, we used olfactory sensory neuronal-like OP6 cells derived from the embryonic olfactory placode cells. Calcium imaging showed that OP6 cells and primary adult OE cell cultures express functional purinergic receptors. We monitored ATP release from OP6 cells and whole adult OE turbinates using HEK cells as biosensors and luciferin–luciferase assays. Constitutive ATP release occurs in OP6 cells and whole adult mouse OE turbinates, and P2X7 receptors mediated evoked ATP release occurs only in turbinates. The mechanisms of ATP release described in the present study might underlie the constant cell turn-over and post-injury neuroregeneration present in adult OE and thus, further studies of these mechanisms are warranted as it will improve our knowledge of OE tissue homeostasis and post-injury regeneration.

中文翻译：

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成年小鼠嗅上皮中 ATP 的组成型和诱发性释放

在成人嗅上皮 (OE) 中，ATP 在恒定的细胞更新和损伤后神经再生中发挥作用。我们之前证明，新生小鼠 OE 中存在组成型和 ATP 诱发的 ATP 释放，是连续细胞更新和损伤后神经再生的基础，并且嘌呤能 P2X 的激活7受体参与诱发释放。我们假设这两种释放都存在于成年小鼠 OE 中。为了研究嗅觉感觉神经元对 ATP 释放的假定贡献，我们使用源自胚胎嗅基板细胞的嗅觉感觉神经元样 OP6 细胞。钙成像显示 OP6 细胞和原代成体 OE 细胞培养物表达功能性嘌呤能受体。我们使用 HEK 细胞作为生物传感器和荧光素-荧光素酶测定来监测 OP6 细胞和整个成年 OE 鼻甲的 ATP 释放。OP6 细胞和整个成年小鼠 OE 鼻甲和 P2X 中发生组成型 ATP 释放7受体介导的诱发 ATP 释放仅发生在鼻甲中。本研究中描述的 ATP 释放机制可能是成人 OE 中存在的恒定细胞更新和损伤后神经再生的基础，因此，有必要对这些机制进行进一步研究，因为它将提高我们对 OE 组织稳态和损伤后神经再生的认识。损伤再生。