Abstract

Tuber borchii forms ectomycorrhiza with oaks, hazel, and pines, including Pinus sylvestris. However, its ectomycorrhiza morphotype with P. sylvestris was not comprehensively described so far, and molecular analyses are missing despite a high danger of misidentification of T. borchii ectomycorrhiza with other closely related and less valuable truffle species. We described for the first time the morphology and anatomy of T. borchii-P. sylvestris ectomycorrhiza using differential interference contrast technique and semi-thin sections in combination with molecular confirmation of identity. Color of ectomycorrhiza is reddish to dark brown, and morphotypes are unevenly but densely covered by warts-bearing pin-like cystidia. All layers of the hyphal mantle are pseudoparenchymatous with outer mantle layer formed of epidermoid cells. T. borchii ectomycorrhiza was identified by a molecular comparison with fruitbodies used for inoculation and its respective ectomycorrhizae. T. borchii has a wide ecological amplitude. To get a better insight in mycorrhization requirements, we investigated growth of P. sylvestris and its ectomycorrhiza infection rate with T. borchii in substrate with different lime content. The mycorrhization of P. sylvestris with T. borchii in the mycorrhization substrate and cultivation in greenhouse conditions was successful, with colonization of P. sylvestris varying between 36.5 and 48.1%. There was no significant correlation of mycorrhization to applied lime contents, and consequently to pH in substrate, while the increased levels of lime improved growth of the P. sylvestris seedlings.

中文翻译：

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块茎 borchii Vittad 的描述、鉴定和生长。不同石灰含量对樟子松菌根幼苗的影响

摘要

鲍基块茎与橡树、榛树和松树（包括樟子松）形成外生菌根。然而，迄今为止，其与P. sylvestris的外生菌根形态类型尚未得到全面描述，并且尽管将T. borchii外生菌根与其他密切相关且价值较低的松露物种误认的风险很高，但仍缺乏分子分析。我们首次描述了T. borchii-P 的形态和解剖结构。使用微分干涉对比技术和半薄切片结合分子身份验证来确定樟子松外生菌根。外生菌根颜色为红至深棕色，形态不均匀，但密布有疣状针状囊孢。菌丝套的所有层都是假薄壁组织，外套层由表皮样细胞形成。通过与用于接种的子实体及其各自的外生菌根进行分子比较来鉴定T. borchii外生菌根。T. borchii具有广泛的生态范围。为了更好地了解菌根形成的要求，我们研究了不同石灰含量基质中樟子松的生长及其外生菌根对鲍氏菌的感染率。樟子松与T. borchii在菌根基质中的菌根化以及在温室条件下的培养是成功的，樟子松的定植率在36.5%至48.1%之间变化。菌根化与施用的石灰含量以及基质的 pH 值没有显着相关性，而石灰含量的增加促进了樟子松幼苗的生长。