Abstract

Chronic psoriasis is a kind of immune-mediated skin illness and the underlying molecular mechanisms of pathogenesis remain incompletely understood. Here, we used small RNA microarray assays to scan the differential expressed RNAs in psoriasis patient samples. The downstream miRNAs and its targets were predicted using bioinformatics analysis from online bases and confirmed using fluorescence in situ hybridization and dual‑luciferase report gene assay. Cell ability of proliferation and migration were detected using CCK-8 and transwell assays. The results showed that a new snoRNA Snora73 was upregulated in psoriasis patient samples. Overexpression of Snora73 significantly increased psoriasis cells viability and migration, while knockdown of Snora73 got the opposite results. Mechanistically, our results showed that Snora73 acted as a sponge for miR-3074-5p and PBX1 is a direct target of miR-3074-5p in psoriasis cells. Furthermore, miR-3074-5p suppressed psoriasis cell proliferation and migration, while PBX1 promoted cell proliferation and migration in psoriasis. Collectively, these findings reveal a crucial role of Snora73 in progression of psoriasis through miR-3074-5p/PBX1 signaling pathway and suggest a potential therapeutic strategy.

中文翻译：

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小核仁 RNA Snora73 通过海绵 miR-3074-5p 和调节 PBX1 表达促进银屑病进展

摘要

慢性牛皮癣是一种免疫介导的皮肤疾病，其发病机制的潜在分子机制仍不完全清楚。在这里，我们使用小 RNA 微阵列检测来扫描银屑病患者样本中差异表达的 RNA。使用在线碱基的生物信息学分析预测下游 miRNA 及其靶标，并使用荧光原位杂交和双荧光素酶报告基因测定进行确认。使用CCK-8和transwell实验检测细胞的增殖和迁移能力。结果表明，一种新的 snoRNA Snora73 在银屑病患者样本中表达上调。Snora73的过表达显着增加了银屑病细胞的活力和迁移，而Snora73的敲低则得到相反的结果。从机制上讲，我们的结果表明 Snora73 作为 miR-3074-5p 的海绵，而 PBX1 是银屑病细胞中 miR-3074-5p 的直接靶标。此外，miR-3074-5p抑制银屑病细胞增殖和迁移，而PBX1促进银屑病细胞增殖和迁移。总的来说，这些发现揭示了 Snora73 通过 miR-3074-5p/PBX1 信号通路在银屑病进展中的关键作用，并提出了一种潜在的治疗策略。