Neutralizing capacity measurement (NCM) of soluble ABH substances (SAS) in plasma was assessed to guide the selection of the appropriate ABO group of fresh-frozen plasma (FFP) for plasma exchange (PE) in blood group O recipients with ABO-incompatible transplantations. Neutralizing capacity was assessed by measuring anti-A and/or anti-B titers in samples comprising one unit of O FFP and 10 O EDTA plasma samples and subtracting the binary logarithm of the titer in each group with a saline dilution. Ten EDTA plasma samples with Lewis b (Le^b) antigen positivity and 10 sets of pooled FFP from each blood group were used as diluents. In O FFP, the NCM values (mean±SD) were 3.4±0.52 (2.6±0.52) and 2.6±0.52 (1.5±0.3) in B and AB for IgM (total antibody) anti-B (both P<0.001), and in the 10 O EDTA plasma samples, they were 3.9±0.88 (3.1±0.88) and 3.2±0.79 (2.4±0.97) for IgM (P=0.0013) and total anti-B (P=0.025), respectively. In vitro analysis revealed that B FFP is more effective than AB FFP in reducing IgM and total anti-B antibody titers in O recipients, regardless of Le^b antigen positivity.

中文翻译：

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AB 与 ABO 特异性血浆对 O 型血受者脱敏的比较分析：一项体外研究。

对血浆中可溶性 ABH 物质 (SAS) 的中和能力测量 (NCM) 进行评估，以指导选择合适的 ABO 组新鲜冷冻血浆 (FFP)，用于 ABO 不相容移植的 O 型血受者的血浆置换 (PE) 。通过测量包含一单位O FFP 和10 O EDTA血浆样品的样品中的抗A和/或抗B滴度并用盐水稀释液减去每组中滴度的二进制对数来评估中和能力。^{使用 10 份具有 Lewis b (Le b} ) 抗原阳性的EDTA 血浆样本和来自每个血型的 10 组汇集的 FFP 作为稀释剂。在 O FFP 中，B 和 AB 中 IgM（总抗体）抗 B 的 NCM 值（平均值±SD）分别为 3.4±0.52 (2.6±0.52) 和 2.6±0.52 (1.5±0.3)（均 P <0.001 ），在10 O EDTA血浆样本中，IgM（P =0.0013）和总抗B（P =0.025）分别为3.9±0.88（3.1±0.88）和3.2±0.79（2.4±0.97）。体外分析表明，无论 Le ^b抗原阳性如何，B FFP 在降低 O 受体中 IgM 和总抗 B 抗体滴度方面比 AB FFP 更有效。