Antimicrobial resistance (AMR) has emerged as one of the most serious worldwide public health issues of the twenty-first century. The expeditious rise of AMR has urged the development of new, natural effective therapeutic strategies against drug-resistant pathogens. Endophytic fungi, which inhabit distinctive environments like endosymbiotic relationships with plants, are gaining interest as alternative reservoirs for novel compounds that exhibit a broad range of chemical diversity and unique modes of action by releasing a variety of secondary metabolites with antimicrobial properties. The objective of the current research was to isolate and identify endophytic fungal species from leaves of Tradescantia pallida and to investigate their antagonistic effects on Multi-Drug-Resistant human pathogens. Endophytic fungus TPL11 and TPL14 showed maximum inhibition in agar plug and agar well diffusion assay. The ethyl acetate crude extract effectively suppressed growth of MRSA (Methicillin-resistant Staphylococcus aureus) ATCC 43300,700699 strains and VRE (Vancomycin-resistant Enterococcus) with the Inhibition zone of 22±0.05, 23±0.11 and 24±0.11mm respectively with minimum inhibitory concentration (MIC) of 3.125µg/mL. Whereas TPL11 fungus revealed antibiosis of 22 ±0.05 and 21±0.15mm against MRSA(ATCC 43300,700699) and 24 ±0.05mm for VRE with MIC of 6.25,3.125 and 1.56μg/mL respectively. The MIC (Minimum inhibitory concentration) index further confirmed that both the extracts were bacteriostatic against MRSA and bactericidal against VRE. The isolates TPL11 and TPL14 were identified as Fusarium oxysporum and Nigrospora sphaerica by 18S rRNA internal transcribed spacer (ITS) sequencing. To our insight, it is the first report to reveal the presence of F.oxysporum and N.sphaerica in T.pallida and their antibacterial activity.

抗菌素耐药性（AMR）已成为二十一世纪最严重的全球公共卫生问题之一。抗菌素耐药性的迅速崛起促使人们开发出针对耐药病原体的新的、天然有效的治疗策略。内生真菌栖息在独特的环境中，例如与植物的内共生关系，作为新型化合物的替代储存库而引起人们的兴趣，这些化合物通过释放多种具有抗菌特性的次生代谢物而表现出广泛的化学多样性和独特的作用模式。本研究的目的是从紫露草叶中分离和鉴定内生真菌物种，并研究其对多重耐药人类病原体的拮抗作用。内生真菌 TPL11 和 TPL14 在琼脂塞和琼脂孔扩散测定中显示出最大的抑制作用。乙酸乙酯粗提物能有效抑制MRSA（耐甲氧西林金黄色葡萄球菌）ATCC 43300、700699菌株和VRE（耐万古霉素肠球菌）的生长，抑菌圈分别为22±0.05、23±0.11和24±0.11mm，最小抑菌浓度 (MIC) 为 3.125μg/mL。而 TPL11 真菌对 MRSA (ATCC 43300,700699) 的抗菌力为 22 ±0.05 和 21±0.15mm，对 VRE 的抗菌力为 24 ±0.05mm，MIC 分别为 6.25、3.125 和 1.56μg/mL。MIC（最低抑菌浓度）指标进一步证实两种提取物对MRSA具有抑菌作用，对VRE具有杀菌作用。通过 18S rRNA 内转录间隔区 (ITS) 测序，分离菌株 TPL11 和 TPL14 被鉴定为尖镰孢 (Fusarium oxysporum)和球形黑孢 (Nigrospora sphaerica) 。据我们了解，这是第一份揭示苍白球菌中尖孢镰刀菌和球形球菌的存在及其抗菌活性的报告。