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Advancements in the loop-mediated isothermal amplification technique for the rapid detection of plant viruses in various crops
Physiological and Molecular Plant Pathology ( IF 2.7 ) Pub Date : 2024-01-20 , DOI: 10.1016/j.pmpp.2024.102229
Ruizuo Fu , Yu Sha , Xiangbin Xu , Song-Bai Liu

The key to success in crop virus management is having the ability to detect pathogens early and accurately. In the last 20 years, loop-mediated isothermal amplification (LAMP) has been widely used in plant disease detection and other fields. It has the characteristics of good specificity, high sensitivity, convenience and speed. Here, we introduce the basic principle of LAMP technology and provide its applications in crop virus detection. Finally, we show the challenges and prospects in the LAMP of crop virus detection. This review will provide a reference for the diagnosis and early prevention of crop viruses by LAMP in the future.

中文翻译:

用于快速检测各种作物中植物病毒的环介导等温扩增技术的进展

作物病毒管理成功的关键是能够及早准确地检测病原体。近20年来,环介导等温扩增(LAMP)已广泛应用于植物病害检测等领域。具有特异性好、灵敏度高、方便、快捷的特点。在此,我们介绍了LAMP技术的基本原理,并给出了其在农作物病毒检测中的应用。最后,我们展示了农作物病毒检测的 LAMP 的挑战和前景。该综述将为今后LAMP对农作物病毒的诊断和早期预防提供参考。
更新日期:2024-01-20
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