Hypervirulent Klebsiella pneumoniae (hvKp) is an emerging pathogen and causes endophthalmitis, liver abscess, osteomyelitis, meningitis, and necrotizing soft tissue infections in both immunodeficient and healthy people. The acquisition of the antibiotic resistance genes of hvKp has become an emerging concern throughout the globe. In this study, a total of 74 K. pneumoniae isolates were collected and identified by VITEK2 and bla_SHV gene amplification. Out of these, 18.91% (14/74) isolates were identified as hvKp by both phenotypic string test and genotypic iucA PCR amplification. The antibiotic susceptibility revealed that 57.14% (8/14) isolates were multidrug-resistant (MDR) and 35.71% (5/14) isolates were extremely drug-resistant (XDR). All the isolates were resistant to β-lactam, β-lactamase + inhibitor groups of antibiotics, and the least resistance to colistin. Of 14 hvKp isolates, all isolates are positive for iroB (100%), followed by iutA (92.85%), peg344 (85.71%), rmpA (57.14%), and magA (21.42%) genes. Among serotypes, K1 was the most prevalent serotype 21.4% (3/14), followed by K5 14.3% (2/14). The most common carbapenemase gene was bla_OXA-48 (78.57%) followed by bla_NDM (14.28%) and bla_KPC (14.28%) which co-carried multiple resistance genes such as bla_SHV (100%), bla_CTX-M (92.85%), and bla_TEM (78.57%). About 92.85% (13/14) of hvKp isolates were strong biofilm producers, while one isolate (hvKp 10) was the only moderate biofilm producer. The (GTG)5-PCR molecular typing method revealed high diversity among the hvKp isolates in the tertiary care hospital. Our findings suggest that MDR-hvKp is an emerging pathogen and a challenge for clinical practice. In order to avoid hvKp strain outbreaks in hospital settings, robust infection control and effective surveillance should be implemented.

高毒力 肺炎克雷伯菌 (hvKp) 是一种新出现的病原体，可引起免疫缺陷者和健康人群的眼内炎、肝脓肿、骨髓炎、脑膜炎和坏死性软组织感染。hvKp 抗生素抗性基因的获得已成为全球关注的新问题。在这项研究中，总共 74  K 。 收集 肺炎链球菌分离株并通过 VITEK2 和bla _SHV 基因扩增进行鉴定。其中，18.91% (14/74) 的分离株通过表型串测试和基因型 iucA  PCR 扩增被鉴定为 hvKp。抗生素敏感性显示，57.14%（8/14）分离株具有多重耐药（MDR），35.71%（5/14）分离株具有极度耐药（XDR）。所有分离株均对β-内酰胺、β-内酰胺酶+抑制剂组抗生素耐药，对粘菌素耐药性最小。在 14 个 hvKp 分离株中，所有分离株均为iroB  (100%)基因阳性， 其次是 iutA  (92.85%)、  peg344  (85.71%) 、rmpA (57.14%)和magA  (21.42%) 基因。在血清型中，K1 是最常见的血清型，占 21.4% (3/14)，其次是 K5，占 14.3% (2/14)。最常见的碳青霉烯酶基因是bla _OXA-48（78.57%），其次是bla _NDM（14.28%）和bla _KPC（14.28%），它们共同携带多个耐药基因，如bla _SHV（100%）、bla _CTX-M（ 92.85%) 和bla _TEM (78.57%)。约 92.85% (13/14) 的 hvKp 分离株是强生物膜产生者，而一种分离株 (hvKp 10) 是唯一中度生物膜产生者。(GTG)5-PCR分子分型方法显示该三级医院的hvKp分离株具有高度多样性。我们的研究结果表明，MDR-hvKp 是一种新兴病原体，对临床实践来说是一个挑战。为了避免 hvKp 菌株在医院环境中爆发，应实施强有力的感染控制和有效监测。